摘要
目的 利用气相色谱法对继发龋牙本质中的有机酸进行定性和定量分析,探讨继发龋形成原因。方法 收集45颗牙齿继发龋牙本质标本,用25g/L偏磷酸溶解并匀化,低温离心,取上清液加入100g/L高碘酸混匀后进样作样品乳酸分析,其他有机酸直接进样上清液分析。结果 大多数继发龋牙本质标本中检测到乳酸、甲酸、乙酸、丙酸和丁酸存在,少数标本中检测到成酸。测得的有机酸含量由高到低依次为甲酸(23.27±4.36)g/L;乙酸(3.65±1.49)g/L;丙酸(2.38±0.48)g/L;乳酸(1.25±1.20)g/L;丁酸(0.89±0.36)g/L和成酸(0.05±0.13)g/L。结论 继发龋牙本质中的有机酸以甲酸、乙酸和丙酸为主,占所有有机酸组成的93％,主要是细菌利用龋损中胶原等内源性物质产生。
Objective To explore the causes of secondary caries by assay of the content of organic acids in secondary caries. Methods Forty-five secondary carious samples were taken from thirty patients aging from 22 to 60 years old. All dentin samples were homogenized in 25g/L metaphosphoric acid solution. The 100g/L periodic acid was added into the supernatant and the mixture was assayed for lactic acid. The other organic acids were assayed by injecting the supernatant directly into the injector. Results Lactate,formate,acetate, propionate and bu-tyrate were found in most secondary caries samples,and valerate in some samples.The mean concentration of each organic acid in the secondary carious samples was as follows:formate(23.27±4.36) g/L,acetate(3.65± 1.49) g/L,propionate(2.38 ± 0.48) g/L,lactate(1.25 ± 1.20) g/L,butyrate (0.89±0.36) g/L,and valerate(0.05±0.13) g/L. Only a little acetate acid was detected in normal dentin.The concentration was(0.0022±0.040) g/L. Conclusion The main organic acids in secondary caries were formate acid,acetate acid and propinate acid,constituting about 93% of all the organic acids in carious dentin.They were produced mainly from endogenous substracte such as collagen.
出处
《口腔医学》
CAS
2003年第3期132-133,共2页
Stomatology
基金
山东省自然科学基金(L2000C02)
关键词
继发龋
牙本质
有机酸
气相色谱法
Secondary caries
Dentin
Organic acid
Gas chromatography
