摘要
本研究选用昆明种小鼠,在妊娠6～14天,每天腹腔给予0、0.2、1.0和5.0mg/kg的V_2O_5,末次给药后15～18小时,分析母体及胚胎细胞的微核和SCE频率。结果:V_2O_5对SCE频率均无显著影响(P>0.05);但诱导了胎肝及母鼠脾脏、骨髓PEC的微核率的显著增加(P<0.05)。这表明,V_2O_5不仅对成体确具诱变性,而且还能透过胎盘,诱发胚胎细胞的染色体损伤。MNT结果还揭示:胎肝和孕鼠脾脏对化学物质的微核诱导作用较骨髓敏感;当MNT结果在界值附近时,最好采用AO染色法。
0.2~5mg/kg V2O5 was intraperitoneally injected into pregnant mice from 6 to 14 days of gestation. The cytogenetic analysis consists sister-chromatid exchange(SCE ) test and micronucleus test ( MNT ) . The results showed that the SCE frequencies in both the fetal livers and maternal bone marrows were not significantly increased ( P>0.05, compare with control ) , while the MN rate of polychromatic erythrocy-tes in fetus and maternal bone marrow and spleen were increased significantly ( P<0.05, compare with control ) . It showed that V2O5 could bring about genetic damage in both the fetal and maternal cells and the fetal liver and maternal spleen were more sensitivity for induction of MN frequencies than maternal bone marrow.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1992年第3期119-122,共4页
Journal of Environment and Health
关键词
钒
微核试验
污染物
测定
Micronucleus test Sister chromatid exchange Vanadium Transplac-ental mutagenesis
