摘要
目的 :观察过氧化物酶体增殖体激活受体 - γ( PPAR- γ)激活对糖基化终产物 ( AGEs)引起的大鼠肾皮质基质金属蛋白酶 - 2 ( MMP- 2 )活性降低的影响。方法 :每日给正常大鼠尾静脉注射AGE-修饰大鼠血清蛋白 ( AGEs组 ) ,其中部分大鼠同时灌胃给予 PPAR- γ激活剂罗格列酮 ( AGEs+ RSG组 ) ,注射天然大鼠血清蛋白 (天然血清组 )和不施加处理的正常大鼠 (空白对照组 )作为对照 ,6周后处死动物 ,检测大鼠肾功能 ,酶谱法分析肾皮质 MMP- 2活性。结果 :与天然血清组及空白对照组比较 ,注射 AGE-修饰大鼠血清蛋白的大鼠尿蛋白含量明显增加 ( P<0 .0 5 ) ,活性形式的MMP- 2活性明显降低 ( P<0 .0 5 ) ,而同时给予罗格列酮的大鼠尿蛋白含量明显降低 ( P<0 .0 5 ) ,MMP- 2活性明显增加 ( P<0 .0 5 )。结论 :PPAR- γ激活可以改善 AGEs引起的肾功能变化 ,减轻AGEs对大鼠肾皮质 MMP- 2活性的抑制 ,提示 PPAR-
Objective: To investigate the effects of peroxisome proliferator activated receptor γ activation on advanced glycation end products (AGEs) reducing matrix metalloproteinase 2(MMP 2) activity in rat renal cortex. Methods: AGE modified rat serum protein (AGE RSP)prepared in vitro was administered intravenously to normal rats with (AGEs+RSG) group or without rosiglitazone (AGEs group), and native rats serum protein was given as negative control (negative group) and normal rats without treatment were as control (control group). Renal function was analyzed by blood BUN, blood creatinine, urine creatinine, urine protein and MMP 2 activity was determineded by zymography. Results: After 6 week treatment,urine protein increased markedly and the activity of active form MMP 2 (62 000) reduced significantly in rats receiving AGE RSP (AGEs group) as compared with those in rats treated with native RSP or in control. However, rats given rosiglitazone (AGEs+RSG group) had normal urine protein content and MMP 2 (62 000) activity. Conclusion:PPAR γ activation can ameliorate the renal function and the decreased MMP 2 activity induced by AGEs, suggesting that PPAR γ activition may increase ECM degradation in diabetic nephropathy.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第3期255-257,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助项目 (39870 312 )
