摘要
目的 探讨青少年载脂蛋白 E( apolipoprotein E,apo E)、apo B基因多态性对冠心病的遗传易感性。方法 应用聚合酶链反应 -限制性片段长度多态性技术 ,对 2 4 4名健康汉族大学生 (冠心病家族史阳性者 10 9人 ,阴性者 135人 )的 apo E、apo B Xba 、apo B3′可变数目串联重复序列 ( variable number oftandem repeat,VNTR)基因型进行分析。结果 阳性组的 e4、x+ 、VNTR- B( hypervariable element,HVE>38)等位基因频率显著高于阴性组 ( P<0 .0 5 ) ,且与血总胆固醇、低密度脂蛋白 -胆固醇、apo B10 0水平升高有显著相关 ( P<0 .0 5 )。结论 apo E的 e4、apo B Xba 的 x+ 、apo B 3′VNTR的 VNTR-
Objective: To investigate hereditary susceptibility to coronary heart disease (CHD) in apolipoprotein E (apo E) and apo B polymorphisms of youths. Methods: Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) was used to analyze apoE, apoB Xba I, apoB 3′ variable number of tandem repeat (VNTR) genotypes for 244 healthy Han students (among them were 109 students with positive CHD family history). Results: The allele frequencies of apo e4, XbaI x+, 3′ VNTR-B(hypervariable element, HVE>38) in the positive group were obviously higher than those in the negative group(P+, 3′ VNTR-B may be the important genetic markers of Han CHD.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2003年第3期241-243,共3页
Chinese Journal of Medical Genetics
基金
广州市东山区科技局资助 (2 0 0 0 - QZ- 2 4)~~
