摘要
目的 探讨洛伐他汀 (Lovastatin ,LOV)对MCF 7细胞增殖、分化功能及细胞形态、结构的影响。方法 LOV处理细胞 1～ 3d后 ,MTT比色法检测细胞增殖功能 ,流式细胞仪分析细胞周期相分布及细胞凋亡率 ,同时观察细胞形态、结构的改变。结果 LOV可导致细胞形态、结构改变 ;细胞增殖抑制 ,生长阻滞于G0 /G1期 ;同时LOV有诱导该细胞向正常细胞分化的趋势 ,但诱导细胞凋亡的作用不明显。结论 LOV可导致MCF 7细胞形态、功能改变 ,细胞增殖受抑、生长阻滞于G0 /G1期 。
Objective To study the effects of lovastatin (LOV) on proliferation & differentiation and morphology of MCF 7 cells. Methods After treated MCF 7 cells with LOV for 1～3d, the proliferation of MCF 7 cells were examined with MTT test and the distributions of cell cycles with FCM assay. Meanwhile the morphological change was observed.Results LOV could change the morphology and ultra structure of MCF 7 cells significantly, inhibit the proliferation of MCF 7 cells, induce differentiation and arrest MCF 7 cells in the G 0/G 1 phase of cell cycle. However, apoptosis in MCF 7 cells treated with LOV was not obvious.Conclusion It suggests that LOV has the capabilities of inducing cellular morphological changes, inhibiting proliferation and arresting MCF 7 cells in G 0/G 1 phase of cell cycle. Meanwhile it induces the differentiation of MCF 7 cells.
出处
《肿瘤防治研究》
CAS
CSCD
2003年第3期210-213,共4页
Cancer Research on Prevention and Treatment
