摘要
本研究根据黄瓜花叶病毒2b基因的保守序列设计引物,利用一步RT-PCR技术对多个不同寄主和不同地理来源的黄瓜花叶病毒分离物的2b基因片段进行扩增,获得了含该基因全长约90％的cDNA片段(300bp)。序列测定与比较分析结果表明,国内不同CMV分离物2b基因片段核酸序列同源性达93％以上,推测的氨基酸序列同源性超过90％,且均属于亚组Ⅰ。
A simple and rapid method for detecting Cucumber mosaic virus (CMV) was developed. CMV 2b gene fragments
(300 bp) of 9 different isolates were successfully amoplified by one step RT-PCR from virus samples prepared by grinding in
an extraction buffer. RT-PCR products of 9 isolates were cloned into pMD18-T vector and 7 of them were sequenced. The
comparison of these sequences revealed that 2b gene fragments of different CMV isolates were highly conservative, and the
identity of nucleotide and amino acid sequences were over 93% and 90%, respectively. It showed that all the Chinese iso-
lates used belong to the subgroup Ⅰ of CMV.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第2期146-150,共5页
Acta Phytopathologica Sinica
基金
国家自然基金(30170643)
人事部回国留学人员项目
