摘要
目的 观察生物分子间相互作用动力学、亲和力及特异性 ,研究C5a与其反义肽的相互作用规律。方法 应用生物分子相互作用实时分析系统IAsysPlus,以反义肽R4为固定相 ,L2和rhC5a为流动相 ,采用FASTplot软件对选择结果优化的反应模式 ,计算各动力学参数值。结果 反义肽R4与配体rhC5a相互结合的KD=6 .6 2× 1 0 -6mol,直线在Y轴上的截距(解离常数 )Kdiss为 0 .0 2 2 5s-1 ;与L2的KD=4 .5 0 8× 1 0 -6mol。结论 生物传感器技术可以用于正义 反义肽之间相互作用的研究中 ,且反义肽R4可与其正义肽。
Objective The kinetics, affinity and specificity between the human C5a anaphylatoxin and it's antisense peptide R4 was observed. Methods The antisense peptide R4 was in immobilization, and the rhC5a a nd the sense peptide (L2) of the C5a (that amino acid sequense is VNNDETCEQRAAR) was in streaming movement. The kinetic rate and equilibrium binding constants f or their interaction by using Biomolecular interaction analysis(BIA). Re sults The antisense peptide R4 can bind to anaphylatoxin C5a and its co rresponding sense peptide L2 selectively. The dissociation equilibrium constants ( K D) between R4 and rhC5a is 6.62×10 -6 mol and the K D betwee n R4 and L2 is 4.508× 10 -6 mol. Conclusion There are biospecific interactions between anaphylatoxin C5a and its anti sense peptide. So it is available to study the interactions between the peptide and it's antise nse peptide by using the optic Biosensor.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2003年第3期224-226,共3页
Immunological Journal
基金
国家自然科学基金资助项目 (39970 330 )
