摘要
应用改良的二步温控法 PCR 技术,检测34例人宫颈癌组织 DNA,发现宫颈癌中 HPV-16 E_6/E_7相关序列存在的阳性率为67.6%(23/34)。结果提示:HPV-16可能在宫颈癌的发生发展过程中起重要作用,采用改良 PCR 技术选择性地检测 HPV-16的转化基因(E6、E7)对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。
The modified two-protocol PCR technique was used for detection of the HPV-16 E_6/E_7-related sequences in 34 Chinese cervical cancers,the positive percentage of HPV-16 E_6/E_7 in cervical cancers, was 67.6%(23/34).This result indicated that HPV-16 might play an important role in the develop- ment and progression of cervical cancers,and use of the modified PCR technique for selective detection the transforming genes(E_6/E_7)of HPV-16 may be helpful with research of cervical carcinogegesis and early finding of cervical cancer patients.
基金
国家"七五"科技攻关专题基金资助
