摘要
目的寻求一种敏感度和特异性高的方法用于输血前和临床诊断检查梅毒螺旋体抗原血清学试验。方法应用TPPA、ELISA、TRUST等三种梅毒螺旋体血清学检测方法,对25例各期梅毒血清标本进行检测,对其敏感性及特异性进行比较。结果25例梅毒血清标本,ELISA法和FPPA法检测阳性率为100％,TRUST法为93％;经稀释法检测,ELISA法较TPPA法敏感。对815例临床标本检测,ELISA方法与TPPA法的检出符合率为100％。结论ELISA法操作简单,结果判断可标准化,特异性强,敏感性高;既可用于过筛试验,又可用于筛检阳性样本的确认。
Objective To find a sensitive and specificity method to detect the Treponemapallidum antibody in the blood before blood transfusion and its application in clinical diagnosis. Methods To detest the antibody of Treponema pallidum in 25 patients sera with TPPA, ELISA and TRUST methods. The sensitivity and specificity of these three methods were compared. Results In the test of twenty-five samples, the positive rate of ELISA and TPPA were 100% , and that of TRUST was 93% , the sensitivity of ELISA was higher than that of TPPA in the dilution test.There was no fail in detecting positive in 815 samples with ELISA and TPPA methods. Conclusion The ELLAS kit is easy to use and has high sensitivity and specificity, which is suitable for sifting tests and confirming tests.
出处
《热带医学杂志》
CAS
2003年第1期75-76,共2页
Journal of Tropical Medicine
