期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

卡维地洛干预心衰对大鼠心肌细胞凋亡和肌浆网钙泵活性的影响 被引量：2

Effects of carvedilol on cardiomyocyte apoptosis and sarcoplasmic reticulum Ca^(2+) AT-Pase activity in rats with congestive heart failure
暂未订购
导出
摘要 目的　从细胞内钙调节和细胞凋亡角度探讨卡维地洛治疗心力衰竭的机制。方法　采用结扎冠状动脉复制心力衰竭大鼠模型 ,对照观察非选择性 β 受体阻滞剂卡维地洛和α1 受体阻滞剂特拉唑嗪干预心衰对左室心肌细胞凋亡率和肌浆网钙泵 (SERCA2a)活性的影响。结果　与对照组 (C组 )相比 ,心衰组 (F组 )SERCA2a活性显著降低 (P <0 .0 1) ,心肌细胞凋亡率显著升高 (P <0 .0 1) ;特拉唑嗪组 (T组 )心肌细胞凋亡率、SERCA2a活性与F组无显著差异 (P >0 .0 5 ) ;卡维地洛剂量依赖地降低心肌细胞凋亡率 ,增高SERCA2a活性。SERCA2a活性与心肌细胞凋亡率显著负相关 (r =-0 .814 ,P <0 .0 0 1) ,与 +dp/dtmax、-dp/dtmax显著正相关 (r =0 .691、0 .64 5 ,P <0 .0 0 1)。结论　SERCA2a活性异常 ,可能参与心力衰竭心肌细胞凋亡的调节。增加SERCA2a活性 ,抑制心肌细胞凋亡 ,可能是卡维地洛治疗心力衰竭的重要机制。卡维地洛的上述效应与α1 受体阻滞作用无关。 Objective To study the mechanism of carvedilol therapy of congestive heart failure. Methods After chronic heart failure model was established by ligation of the left coronary artery in rats, rats were treated with carvedilol and terazosin. Then hemodynamic parameters, activity of sarcoplasmic reticulum(SR) Ca 2+ pump(SERCA 2a ) and the rate of cardiomyocyte apoptosis were determined. Results Compared with those of the control group( group C), the activity of SR Ca 2+ pump in the heart failure group(group F) decreased( P <0.01) but the rate of cardiomyocyte apoptosis increased significantly( P <0.01). There was no significant difference between terazosin treated group(group T) and group F( P >0.05). Carvedilol intervention reduced the rate of cardiomyocyte apoptosis, but enhanced the activity of SR Ca 2+ pump significantly in dose dependent manner. The activity of SR Ca 2+ pump was negatively correlated with the rate of cardiomyocyte apoptosis( r =-0.814, P <0.001) but positively with +dp/dt max and -dp/dt max ( r =0.691, 0.645, respectively, P <0.01). Conclusion Abnormality of SERCA 2a activity may contribute to the regulation of cardiomyocyte apoptosis in the progress of heart failure. The inhibition of myocyte apoptosis and enhanced activity of SERCA 2a may be effective mechanisms of carvedilol in the treatment of congestive heart failure. The beneficial effects of carvedilol on SERCA 2a activity and cardiomycyte apoptosis are not related to the blocking effect of α 1 adrenergic receptor.
作者 耿召华 李隆贵 吴强
机构地区 第三军医大学附属新桥医院全军心血管内科中心
出处 《第三军医大学学报》 CAS CSCD 北大核心 2003年第9期770-773,共4页 Journal of Third Military Medical University
关键词 心力衰竭 肌浆网钙泵 凋亡 P-受体阻滞剂 congestive heart failure sarcoplasmic reticulum Ca 2+ pump apoptosis β blocker
分类号 R322.11 [医药卫生—人体解剖和组织胚胎学] R541.6 [医药卫生—心血管疾病]
  • 相关文献

参考文献11

  • 1耿召华,李隆贵,黄坚,胡友勇,耿建萌,何作云,祝善俊.培哚普利对慢性心功能不全大鼠心肌肌浆网Ca^(2+)泵功能的影响[J].第三军医大学学报,2000,22(9):875-878. 被引量：1
  • 2耿召华,李隆贵,黄坚,胡友勇,耿建萌.培哚普利对心衰大鼠心肌肌浆网Ca_(2+)泵活性和Ca_(2+)释放通道密度的影响[J].第三军医大学学报,2000,22(12):1145-1147. 被引量：4
  • 3Packer M, Bristow M R, Cohn J N, et al. For the US carvedilol heart failure study group. The effect of carvedilol on morbidity and mortality in patients with chronic heart failure[J]. N Engl J Med, 1996,334(21) : 1349 -1355.
  • 4MERIT-HF study group. Effect of metroprolol CR/XL in chronic heart failure: metoprolol CR/XL randomized intervention trial in congestive heart failure (MERIT-HF) [J]. Lancet, 1999,353(9196):2001-2007.
  • 5Kim Y K, Kim S J, Kramer C M, et al. Altered excitation-contraction coupling in myocytes from remodeled myocardium after chronic myocardial inffarction[J]. J Mol Cell Cattdial, 2002,34(1):63-73.
  • 6Hasegawa K, Iwai-Kanai E, Sasayama S. Neurohormonal regulation of myocardial cell apoptosis during the development of heart failure[J]. J CellPhysiol, 2001,186( 1 ) : 11 - 18.
  • 7Singh K, Communal C, Sawyer D B, et al. Adrenergic regulation of myocardial aoootosis[J]. Cardiovasc Res, 2000,45(3) :713 - 719.
  • 8Drexler H, Depenbuseh J W, Truog A G, et al. Effocts of diltiazern on cardiac function and regional blood at rest and during exercise in a conscious rat preparation of chronic heart failure (myocardial iiffnretion) [J]. Circula-tion, 1985,71(6) : 1260 - 1270.
  • 9Afzal N, Dhalla N S. Different changes in left and fight ventfieular SR calcium transport in congestive heart failure[J]. Am J Physiol, 1992,262(4) :H868- H874.
  • 10Houser S R, Piacentino V, Weisser J. Abnormalities of calcium cycling in the hypertrophied and failing heart[J]. J Mol Cell Catrdiol, 2000,32(9):1595-1607.

二级参考文献10

  • 1陈兰英 陈曦.两亲化合物与肌浆Ca ATPase作用的研究Ⅱ.影响酶活力因素的初步探讨[J].Acta Biochinica et Biophysica Sinica（生物化学与生物物理学报：英文版）,1987,19(6):437-445.
  • 2陈兰英，生物化学与生物物理学报，1987年，19卷，6期，437页
  • 3Macfden R J.Role of the circulating and tissue-based renin-angiotensin system in the development of heart failure: implications for therapy[].Cardiology.1993
  • 4Yoshiyama N,Takeuchi K,Hanatani A,et al.Differences in expression of sarcoplasmic reticulum Ca2+-ATPase and Na+-Ca2+exchanger genes between adjacent and remote noninfarcted myocardium after myocardial infarction[].Journal of Molecular and Cellular Cardiology.1997
  • 5Hasenfuss G.Alterations of calcium-regulatory proteins in heart failure[].Cardiovascular Research.1998
  • 6Drexler H,Depenbusch J W,Truog A G,et al.Effects of diltiazem on cardiac function and regional blood at rest and during exercise in a conscious rat preparation of chronic heart failure(myocardial infarction)[].Circulation.1985
  • 7Afzal N,Dhalla N S.Different changes in left and right ventricular SR calcium transport in congestive heart failure[].American Journal of Physiology.1992
  • 8Barry W H,Bridge J H B.Intracellular calcium homeostasis in cardiac myocytes[].Circulation.1993
  • 9Hisamatsu Y,Ohkusa T,Kihara Y,et al.Early changes in the functions of cardiac sarcoplasmic reticulum in volume-overloaded cardiac hypertrophy in rats[].Journal of Molecular and Cellular Cardiology.1997
  • 10Spinale F G,Holzgrefe H H,Mukherjee R,et al.Angiotensinconverting enzyme inhibition and the progression of congestive cardiomyopathy : effects on left ventricular and myocyte structure and function[].Circulation.1995

共引文献3

同被引文献49

  • 1吴红栋,刘俭雄,李军,石理.钙拮抗剂对原发性高血压患者肾功能的影响[J].邯郸医学高等专科学校学报,2004,17(5):382-383. 被引量：1
  • 2李春元.噻嗪类利尿剂预防中风的有益作用[J].世界临床药物,2005,26(1):52-54. 被引量：2
  • 3陈楠,陈佳韵.ACEI和ARB的肾脏保护机制、临床研究及其应用[J].中华医学信息导报,2005,20(5):14-14. 被引量：32
  • 4Akhtar S,Barash PG.Perioperative use of beta-blockers:past,present,and future [J].Int Anesthesiol Clin,2002,40(2):133-157.
  • 5Dzimiri N.Regulation of beta-adrenoceptor signaling in cardiac function and disease [J].Pharmacol Rev,1999,51:465-501.
  • 6Guimaraes S,Moura D.Vascular adrenoceptors:an update [J].Pharmacol Rev 2001,53:319-356.
  • 7Dhalla NS,Temsah RM,Netticadan T.Role of oxidative stress in cardiovascular diseases [J].J Hypertens,2000,18(6):655-673.
  • 8Andona P,Karne R,Ghanim H,et al.Carvedilol inhibites reaction oxygen species generation by leukocytes and oxidative damage to amino acids[J].Circulation,2000,101:122-124.
  • 9Oettl K,Greilberger J,Zangger K,et al.Radical-scavenging and iron-chelating properties of carvedilol,an antihypertensive drug with antioxiodative activity[J].Biochem Pharmacol,2001,62(2):241-248.
  • 10Cargnoni A,Ceconi C,Bernocehi P,et al.Reduction of oxidative stress by carvedilol:role in maintenance of ischaemic myocardium viability[J].Cardiovasc Res,2000,47(3):556-566.

引证文献2

二级引证文献18

第三军医大学学报
2003年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部