摘要
目的 分离、克隆约氏疟原虫动力素蛋白(Plasmodium yoelii dynamin-like protein,PyDyn)基因,并用其在原核表达的蛋白结构域产物做候选疫苗,研究其免疫保护性。方法 应用约氏疟原虫基因组数据库,设计特异引物,用RT-PCR法从约氏疟原虫红内期mRNA扩增PyDyn基因编码区;分析其序列特性;原核表达约氏疟原虫动力素结构域蛋白、IFA分析其免疫原性。结果 从鼠约氏疟原虫mRNA扩增的PyDyn基因编码区是一个全长为2433 bp的cDNA克隆,编码811个氨基酸的蛋白肽链,具有典型的动力素蛋白家族的结构特性。该基因GeneBank登录号为AF458071。IFA表明该虫源性结构蛋白(PyDyn)的第1、3、5结构域的抗血清可以识别约氏疟原虫感染的红细胞。结论 经分离、克隆和表达的新PyDyn有望成为疟疾疫苗的候选抗原分子。
Objective To identify and clone a new full ORF gene of PyDyn (Plasmodium yoelii dynamin-like protein), and examine the protection of their expression products. Method Using the P. yoelii Genome technology and RT-PCR. Results The full ORF gene of PyDyn was amplified from mRNA of the erythrocytic stage of P. yoelii, three domains of PyDyn were expressed in E.coli., and the fairly positive immunogenicity of them was showed by IFA. The full ORF gene of PyDyn was 2 433 bp and encode 811 amino acids. Its Gene Rank access number is AF458071. PyDyn belongs to the dynamin-like protein family according to its property. Conclusion The new full ORF gene of PyDyn is obtained and identified; their expressed domains are probably new candidates for malaria vaccine.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
2003年第2期176-180,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金(30170876)资助
关键词
约氏疟原虫
动力素蛋白
疫苗
Plasmodium yoelii
dynamin-iike protein
vaecine
