摘要
目的 从细胞水平探索细胞骨架在将机械应力刺激信号转导进入细胞中并产生应答反应的作用机制。方法 在建立力学刺激细胞培养系统的基础上 ,对体外分离培养的原代大鼠颅骨成骨细胞分别施加 1.2或1.9N/m2 的流动剪切力 ,激光共聚焦显微镜下观察流动剪切力作用后成骨细胞细胞骨架的改变。结果 1.2N/m2 的流动剪切力作用 1h后 ,受力细胞及其细胞突起沿流动方向一致被拉长。细胞中央微丝呈束状排列 ,荧光标记强度明显增强。去除剪切力继续培养 2 4h后 ,细胞恢复无方向的排列 ,细胞胞浆微丝重新分布于细胞的周围。 1.9N/m2 的流动剪切力作用 1h后 ,受力细胞发生明显的皱缩 ,细胞脱落明显 ,胞浆微丝出现解聚消失现象。继续培养 2 4h后 ,细胞形态及微丝无明显恢复。结论 细胞骨架是成骨细胞受到流动剪切力后的一个机械刺激感受体 。
Objective To study the mechanism of cytoskeletal by which fluid shear stress regulated the physiological function of osteoblastic cells.Methods The isolated rat primary osteoblastic cells had been exposed to fluid shear stress 1.2 or 1.9 N/m 2 for 1h and released in the flow chamber. The morphological change and cytoskeletal were studied right after releasing and 24 hours later. Results After 1h exposed to 1.2 N/m 2, the cells rearranged, the cells were elongated and the filaments were recomposed following the flow direction. All resumed 24 hrs later. But when exposed to 1.9 N/m 2 , the cells were crimpled, and the filaments were disentwined. All maintained 24 hrs later. Conclusions There is correlation between the morphological change and cytoskeletal. The cytoskeletal may be a mechasensor which transduced the fluid shear stress into osteoblastic cells and then regulated the physiology of cells.
出处
《广东牙病防治》
2003年第2期83-85,T001,共4页
Journal of Dental Prevention and Treatment
基金
国家自然科学基金资助项目 (编号 :3 960 0 167)
