摘要
应用反转录_聚合酶链反应 (RT_PCR)对猪瘟进行诊断应用研究。应用RT_PCR对来自广西不同地区的 135份疑似猪瘟病料进行检测 ,84份诊断为阳性 ,阳性率 6 2 .2 %。从百色、柳州地区等采集的健康猪扁桃体和淋巴结共 2 76份 ,经RT_PCR检测 ,37份为阳性 ,阳性率为 13.4 %。其中健康猪扁桃体带毒较高 ,2 4 6份扁桃体中有 35份阳性 ,占 14 .2%。采自柳州健康猪的 2 6份淋巴结材料全为阴性 ,只有邕宁县的 1份健猪淋巴结阳性。结果表明 。
Reverse transcriptase polymerase chain reaction (RT_PCR) was applied to diagnose hog cholera (HC). The result showed that 84 of 135 clinical samples from different places in Guangxi were positive, the rate of positive was 62.2 %. The samples of tonsils and lymph nodes that were collected from healthy pigs from Baise and Liuzhou have been detected. Among 276 tonsil samples of healthy pigs, 37 samples were positive, the rate of positive was 13.4 %. But only one lymph node sample of healthy pig from Yongning indicated positive. In which the samples of tonsil from healthy pigs showed higher rate of positive, 35 of 246 tonsil samples were positive. The rate of positive was 14.2 %. According to the results, RT_PCR is an useful method that can be applied to diagnose HC clinically.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2003年第3期219-222,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
广西大学博士科研启动基金 (X9830 3)
广西区教育厅资助项目 (T9810 6 )
关键词
猪瘟
反转录-聚合酶链式反应
诊断
hog cholera (HC)
reverse transcriptase polymerase chain reaction (RT_PCR)
diagnosis
