摘要
目的依据颞下颌关节(TMJ)髁突软骨内成骨及关节凹膜内成骨的生长模式,观察TMJ在下颌前导位时的新生血管反应,在分子生物学水平探究新生微细血管增殖在TMJ骨组织改建中的作用。方法35天雌性Sprague-Dawly大鼠随机分成5个实验组和5个对照组。实验组动物戴用咬合前导功能性矫正器(BJA),造成持续的下颌前导。实验组和对照组动物分别在第3、7、14、21、30天时被处死。取下TMJ组织,石蜡包埋,切片,用于免疫组化分析。使用单克隆抗体EN7/44对新生血管内皮细胞进行免疫染色,评价新生血管形成。采用LeicaQwin图像辅助分析系统,对新生血管进行定量分析。结果(1)连接髁突和关节窝后面的结缔纤维组织中,标志新生血管的免疫染色实验组比对照组平均高200%(P<0.01);(2)在髁突和关节窝骨组织的新生血管免疫染色中,实验组比对照组分别高103%和163%(P<0.01)。结论下颌前导后TMJ软硬组织内新生血管形成增多,这可能是导致TMJ新骨增加的重要机制之一。
Objective Neovascularization is proved to be closely correlated with ossification.This study was designed to determine the biochemical path through which the new bone formation is enhanced in TMJ during mandibular protrusion.Methods SD Rats were fitted with bite-jumping appliances to allow for an adequate advancement of the mandible.The animals were sacrificed3,7,14,21and30days,respectively,after placement of the appliances.Slides with TMJ tissue were prepared for biochemical procedure.Immunohistochemical approaches were adopted to examine neovascularization in TMJ by immuno-localizing the newly formed endothelial cells.Computer-assisted image analysis system was applied to quantify the positive immunoreaction.Results Neovascularization in connective tissue close to the condyle was200%higher in the experimental groups than that in the control,and,within the bony tissue of the condyle,neovascularization in experimental animals was found to be103%higher than that of the control.Conclusion Increased neovascularization trig-gered by mandibular protrusion might intensify new bone formation in condyle and glenoid fossa and finally lead to a growth adaptation of TMJ to bite-jumping therapy.<
出处
《上海口腔医学》
CAS
CSCD
2003年第2期115-119,共5页
Shanghai Journal of Stomatology
基金
上海市教委基金(99B09)
香港大学研究基金(10201953)
