摘要
本文报道用葡聚糖为中介体制备抗胃癌单抗MGb_2与平阳霉素的结合物,经测算每克分子抗体可携带60～70克分子的药物。在结合物制备过程中抗体活性无明显丧失,结合物能与靶细胞特异结合。体外细胞毒试验证实,结合物对靶细胞(食管癌细胞ECA—109)具有较强的选择性杀伤作用,优于游离平阳霉素及无关抗体结合物;对非靶细胞则毒性明显减弱。提示该免疫结合物可能会成为一种较好的抗体导向化疗药物。
In the present study,pingyangmycin (PYM) was linked covalently to the murine monoclonal antibody MGb_2 for human gastric cancer via a dextran (DEX)bridge. The molar ratio of MGb_2: DEX: PYM was 1: 3: 65±5 in the conjugate. It was confirmed by indirect immunofluorescent staining that the immunological activity of MGb_2 in the conjugate was well preserved. The conjugate can well bind to tumor target cells when the concentration of MGb_2 in the conjugate was 1×10^(-10)mol/L. In vitro study indicated that the conjugate showed stronger selective cytotoxicity against tumor target cell lines (ECA-109) than free PYM and the normal mouse IgG-DEX-PYM conjugate (P<0. 01) and less cytotoxicity against nontarget cell lines (HEP-2) than free PYM(P<0. 05) in the same study. The results suggested that MGb_2-DEX-PYM conjugate is a better antibody-guided agent.
出处
《河南肿瘤学杂志》
1992年第1期12-14,共3页
Henan Journal of Oncology
关键词
单克隆抗体
平阳霉素
食管肿瘤
monoclonal antibody
Bleomycins
esophagus carcinoma
