摘要
目的 探讨结核杆菌耐利福平 (RFP)检测基因芯片在结核病诊断及其耐药性检测中的应用价值。方法 采用结核杆菌耐RFP芯片对 35株RFP耐药的临床分离菌株 ,10 2例肺结核患者、2 7例非结核病的其他患者的痰标本中的结核杆菌及其RFP耐药性进行了检测 ,基因芯片检测结果与痰涂片、细菌培养及结核杆菌标准化药敏试验结果比较。结果 结核杆菌耐RFP检测芯片检测 35株耐药株 ,有 33株判定为RFP耐药株 ,与传统药敏试验结果的符合率为 94 .2 9%。对 2 7份其他患者的痰液标本进行结核杆菌检测 ,特异性为 92 .5 9%。对 10 2份结核患者痰标本中结核杆菌进行检测 ,痰涂片法阳性率 35 .2 9% (36 /10 2 ) ,细菌培养法阳性率 2 8.4 3% (2 9/10 2 ) ,基因芯片法阳性率 77.4 5 %(79/10 2 )。传统的药敏试验报告 10 2份痰标本仅 2 9份培养出结核分支杆菌 ,其中 8份RFP耐药株 ,而基因芯片法检测 10 2份痰标本中发现 2 0份耐RFP结核杆菌。主要基因突变位点为 5 31、5 2 6和5 16。结论 采用结核杆菌耐RFP检测芯片检测结核杆菌及RFP耐药性具有快速简便、特异性高的特点。
Objective To evaluate the gene-chip detecting rifaman-resistance Mycobacterium tuberculosis applied in TB diagnosis and drug-resistant dectection. Methods Mycobacterium tuberculosis and rifaman-resistant strains among 35 rifaman-resistance isolated strains and 102 sputa specimens from TB patients,27 sputa specimens from other patients were examined the gene-chips. Results obtained were compared with sputum examination, bacteriological culture and standard drug susceptibility test of Mycobacterium tuberculosis . Results Thirty-five rifaman-resistance strains were detected by gene-chips and 33 were identified as rifaman-resistance strains and the concordance with the traditional drug susceptibility test of Mycobacterium tuberculosis was 94.29 %. Twenty-seven sputa specimens from other patients were examined Mycobacterium tuberculosis by the gene-chips, 2 were positive, the detection specialty was 92.59 %. Using three methods detecting Mycobacterium tuberculosis among 102 sputa specimens the positive rate respectively was, sputum examination 35.29 % (36/102), bacteriological culture 28.43 % (29/102), gene-chip 77.45 % (79/102). Among 102 sputa specimens only 29 examined Mycobacterium tuberculosis by the traditional drug susceptibility test and 8 were rifaman-resistant strains. While using gene-chip, there were 20 among 102 sputa specimens identified as rifaman-resistance strains. Among total 55 rifaman-resistance strains detected by the gene-chips, the most frequent mutations were those associated with codon 531 (23 of 55; 41.8 %), 526(15 of 55; 27.27 %) and 516(9 of 55; 16.36 %). Conclusion Results showed that this was a rapid, simple and highly specific method when using gene-chip to detect Mycobacterium tuberculosis and rifaman-resistant strains.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2003年第5期385-388,共4页
Chinese Journal of Epidemiology
基金
广西科技厅资助项目 (桂科攻 0 2 3 5 0 2 4 2 7)
