摘要
试验优选了检测鸡血清中多杀性巴氏杆菌抗体的微量凝集试验条件,改进了抗原的制备方法。结果表明,改进后的微量凝集试验具有良好的敏感性和特异性,凝集图案清晰,易于观察;试验还证实了鸡血清中的抗体凝集效价与其对强毒菌株攻击的保护作用一致,初步认为,鸡霍乱抗体的免疫临界线为1:28。
A micro—agglutination test(MAT)for detecting antibody against
fowl cholera in chicken serum was developed by increasing the concentration
of antigen and straining the bacterium and the assay was performed on mic-
ro—agglutination plate of 96 wells.The results showed that the MAT was se
nsitive and special,the agglutinating pattern was obvious for observing and
the MAT titer of humoral antibody against fowl cholera correlated signific-
antly with protection against Pasteurella Multocida challenging.
出处
《河南农业大学学报》
CAS
CSCD
1992年第1期48-51,共4页
Journal of Henan Agricultural University
关键词
微量凝集试验
抗体
鸡霍乱
凝集原
micro—agglutination test
humoral antibody
fowl cholera
agglutinin
