摘要
目的 探讨耐苯妥英钠 戊巴比妥钠 (PHT PB)点燃模型中后放电阈值作为耐药性癫判别指标的可靠性。方法 采用PHT、PB对 40只杏仁核点燃鼠进行耐药筛选 ,以后放电阈值 (ADT)来判断点燃鼠对PHT和PB的敏感性 ,分为药物有效组和耐药组 ,每组含点燃大鼠 5～ 6只 ,同步测定大鼠脑部P 糖蛋白 (PGP)的表达。结果 药物有效组大鼠ADT较耐药组明显增高 ,但耐药组大鼠脑组织广泛区域的PGP表达较药物有效组显著增强。结论 耐PHT、PB点燃鼠阈后电位的变化与其耐药机制有关 ,可将其作为判别癫鼠耐药的指标 ,并可利用其建立难治性癫模型 。
Objective To study the reliability of after discharge threshold (ADT) in differentiating the drug-resistant rat in rapidly kindled epilepsy model. Methods Models of epilepsy were produced in rapidly kindled rats and anti-epileptic drug PHT and PB were used to discriminate different drug response groups, which were PHT-PB responder and non-responder. ADT was selected as the criterion to judge the kindled rats' sensitivity to PHT and PB, at the same time, the expression of P-glycoprotein (PGP) in rats' brain was tested.Results PHT and PB could improve the ADT significantly in kindled rats showing response to PHT and PB as compared with the non-responder group(P<0.05). Increased PGP staining in specimens of brain from PHT-PB non-responders was found as compared with that from the responders (P< 0.01).Conclusion ADT is related to the mechanism of drug resistance in PHT-PB non-response kindled rats. ADT can be used as the standard for discriminating the drug resistance, which is helpful in constructing models of refractory epilepsy.
出处
《上海医学》
CAS
CSCD
北大核心
2003年第4期268-270,共3页
Shanghai Medical Journal
