摘要
目的 探讨同型半胱氨酸 (HCY)对内皮细胞中单核细胞趋化蛋白 1 (MCP 1 )表达的影响。方法 使人脐静脉内皮细胞 (HUVECs)暴露于不同浓度的HCY ,孵育 8h后 ,用免疫细胞化学和细胞ELISA方法检测其单核细胞MCP 1的表达。结果 培养的HUVECs能表达单核细胞MCP 1。免疫细胞化学显示 ,0 .1mmol·L- 1 、0 .5mmol·L- 1 和 1mmol·L- 1 HCY组单核细胞MCP 1表达的平均吸光度值分别为 0 .0 789± 0 .0 0 81、0 .0 948± 0 .0 383和 0 .1 1 0 7±0 .0 1 71 ,均显著高于对照组 (0 .0 4 1 1± 0 .0 0 4 6 ,P <0 .0 1 )。细胞ELISA显示 ,上述各实验组单核细胞MCP 1表达的吸光度值分别为 0 .0 4 4 2± 0 .0 0 31、0 .0 4 94± 0 .0 0 39和 0 .0 51 8± 0 .0 0 2 8,对照组为 0 .0 396± 0 .0 0 53 ,方差分析表明 ,各组间均存在显著性差异 (F =58.38,P <0 .0 1 )。结论 培养的HUVECs能低水平表达单核细胞MCP 1 ,HCY能诱导其表达较高水平的单核细胞MCP 1。
Objective To investigate the effects of homocysteine (HCY) on the expression of monocyte chemoattractant protein?1 (MCP?1) in endothelial cells. Methods After exposure of the cultured human umbilical vein endothelial cells (HUVECs) to HCY at increasing concentrations for 8?h, the MCP?1 protein expression was determined by immunocytochemistry and cell ELISA.Results Cultured HUVECs could express MCP?1. Immunocytochemistry showed the mean absorbance values of MCP?1 protein expressions (0.0789±0.0081?0.0948±0.0383 and 0.1107±0.0171) respectively in the experimental groups (0.1?mmol·L -1 , 0.5?mmol·L -1 and 1?mmol·L -1 HCY for 8h) , which were much higher than those of the control group (0.0411±0.0046,P<0.01). Cell ELISA demonstrated the absorbance values in the experimental groups mentioned above were 0.0442±0.0031?0.0494±0.0039 and 0.0518±0.0028 (control group 0.0396±0.0053), respectively. Analysis of variation proved a significant difference between groups(F=58.38, P<0.01).Conclusions Cultured HUVECs could express MCP?1 at a low level, and HCY may be able to induce a higher expression of MCP?1 in HUVECs.
出处
《新乡医学院学报》
CAS
2003年第2期86-88,共3页
Journal of Xinxiang Medical University
