摘要
目的 为确定由PCR技术在小儿慢性分泌中耳炎中耳分泌液中检测到的细菌DNA是否来自于活的细菌。方法 观察中耳分泌液对DNA粘度和DNA酶消化DNA作用的影响。结果 中耳分泌液和DNA的粘度是稳定的 ,中耳分泌液不降解DNA ,DNA酶迅速消化DNA ,中耳分泌液显著抑制DNA酶消化DNA的活性。结论 应用PCR技术在大多数中耳分泌液中检测到的细菌DNA可能不是来自于活的细菌 。
Objective To determine whether or not the bacterial DNA which was detected by PCR comes from viable bacteria.Methods Observe the effection of middle ear effusion(MEE) on DNA viscosity and enzymatic digestion of DNA.Results The middle ear effusion and DNA are stable and DNase 1 rapidly digests DNA,The effusion does not seem to degrade DNA.The middle ear effusion signifcantly inhibits DNase 1.Conclusions Middle ear effusion provides an inhibition of the enzymatic digestion of purified DNA.Thus any DNA found in effusion by PCR techniques could well be fossilized remains and chronic otitis media with effusion may not be the bacterial infection that recent studies have suggested.
出处
《中国医师杂志》
CAS
2003年第4期468-469,共2页
Journal of Chinese Physician
关键词
中耳分泌液
特异性粘度
DNA
小儿
Middle ear effusion
Otitis media with effusion in children
Specific viscosity
DNA
