摘要
本文采用抽滤灭菌常规培养的方法,以小麦科冬58、欧柔和高原602的花药为材料进行离体培养,研究了影响小麦花药培养效果的几个因素,实验结果表明:(1)在培养基中加入一定浓度的甘露醇不仅能提高愈伤组织诱导率,而且还能大幅度提高愈伤组织的质量,从而提高再生植株的诱导频率,降低白苗率;(2)山梨醇的浓度在20-100mM范围内均可提高绿苗产率。愈伤组织诱导率则随着山梨醇浓度和其材料基因型的不同而效果各异;(3)多效唑对小麦花药培养的反应比较复杂,但分化和壮苗时加入多效唑可使绿苗根系粗壮、发达从而提高其移栽成活率。
The factors affecting the effects of anther culture in vitro of Triticum aestivum cv. Kedong 58, Orofen and Gaoyuan 602 were studied. The results indicated that: a)manittol not only increased the callus induction rate but also improved the status of calli induced. The medium with mannitol produced higher frequency of regenerated plantlets and lower frequency of albino plantlets;b) 25-100 mM sobitol increased the frequency of regenerated plantlets, but the induction rates of calli differed with the differences of genotypes and concentrations of sobitol; c) The responses of Bio-MET strengthened the roots of green plants and gave more survival seedlings transplanted.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1992年第3期17-20,共4页
Journal of Hebei Agricultural University
关键词
小麦
花药培养
愈伤组织
诱导率
Triticum aestivum
anther culture
callus
induction rate
