摘要
目的 :克隆变形链球菌葡聚糖结合蛋白B(glucanbindingproteinB ,GbpB)的编码区基因片段。方法 :提取变形链球菌基因组DNA ,采用PCR方法 ,以特异性引物扩增GbpB编码区基因片段 ,并克隆到pBluescriptKS载体 ,筛选阳性克隆进行序列测定。 结果 :PCR扩增到一特异性的约 1 30 0bp的片段 ,克隆后筛选出阳性克隆进行序列分析 ,测定结果表明与国外已发表的序列完全一致。结论 :利用分子生物学技术能成功克隆目的基因 。
AIM:To clone and sequence the glucan bindin g protein B (GbpB) gene of S.mutans. METHODS:The bacteri al chromosomal DNA was isolate from S.mutans Ingbritt and the specific gene fragment was obtained by PCR with two gene specific primers.The segment was ins erted into pBluescript KS vector and the result plasmid was transformed into DH5 α.The positive clone was sequenced.RESULTS:The 1 300 bp spec ific fragment was obtained. The sequence of the gene encoding GbpB was consisten t with those of the references published.CONCLUSION:PCR techni que is effective method to clone the gene encoding GbpB from S.mutans.The re sult will help us to further studies about express and function of GbpB.
出处
《牙体牙髓牙周病学杂志》
CAS
2003年第4期177-179,共3页
Chinese Journal of Conservative Dentistry
基金
高等学校骨干教师资助计划资助项目
全军"十五"计划基金重点课题 ( 0 1Z0 89)
