摘要
大肠杆菌108(pPAHD1)发酵液经离心沉淀,蔗糖高渗处理得到青霉素酰化酶粗品,再经苯乙酰胺-Sepharose4B疏水层析和DEAE-Cellulose DE-52分离纯化,得到可用于结晶的青霉素G酰化酶。在55％饱和度硫酸铵-0.05mol/LPBS,pH7.5条件下批量静置得到该酶晶体。
The crude penicillin G acylase were extracted from E.coli 108 cells by centrifuged and treated with high osmotic shock . Further purified by hydrophobic interaction chromatography with Phenylacetamide-Sepharose 4B and DEAE-Cellulose DE52. After that we got a high purity penillin G acylase to crystallize .Using bath method the crystals of the enzyme were grown from 55% saturated ammonium sulfate in 0.05 mol / L phosphate buffer, pH7.5
出处
《河北大学学报（自然科学版）》
CAS
1992年第3期57-61,共5页
Journal of Hebei University(Natural Science Edition)
