摘要
目的 :本文应用非脱钙骨切片技术研究皮质骨在lmm和 10mm骨缺损修复过程中的变化及其在骨修复中的作用。方法 :新西兰大白兔 30只 ,分成l、2、3、4、6周组 ,每组再分成lmm及 10mm 2组 ,每组 3只 ,右侧分别为 1、10mm骨缺损侧 ,左侧为对照。制作非脱钙骨切片 ,组织学观察骨缺损愈合过程中皮质骨结构变化。结果 :组织学观察显示 ,两骨缺损模型修复过程的早期 ,皮质骨断端各表面均出现了骨融解现象 ,皮质骨陷窝内的骨细胞被溶解并释放出 ,与周围骨缺损修复组织联系紧密。 4周后 ,在lmm骨缺损模型中 ,在外骨痂附近的皮质骨部位有许多的改建与再建的哈佛氏系统单位 ,皮质骨内的再建结构与外骨痂及连接骨痂内的再建结构紧密相连 ;而 10mm模型中 ,皮质骨无明显变化。结论 :伴随骨修复过程 ,骨缺损两端皮质骨发生特征性改变 ,在骨愈合再生期 ,可为骨修复过程提供促骨修复细胞及物质 ;而在骨愈合的改建期 ,也参与各种骨痂的改建过程。因此 ,皮质骨是骨修复过程的再生及重建过程中重要影响因素。
Objective:To study the change inside cortical bone in two different size bone defect of animal models during bone defect repair process. Method: The study was performed on 30 young adult New Zealand white rabbits, six to eight months old. All animals received osteotomy at right side radius without operation on the left side, which served as control. The animals were divided into two groups, l mm defect group and 10 mm defect group. Defects, 10-mm long and 1-mm long, were created by electric saw, with respects to two different group. Animals in each group were euthanized at 1, 2, 3, 4, and 6 weeks, from the start of the experiment. Specimens were embedded in methylmethacrylate. Five-micron-thick sections were cut through the middle coronal plane of the samples using sliding microtome for hard tissues (Leica 2155). Slides were stained with tuludin blue. Result: In the early stage of bone defect healing process, 1-3 weeks after operation, resorptive changes were found inside of cortical bone around the bone defect in both models, with close relationship with the bone healing tissue. After 4 weeks, in the lmm defect model, a lot of Bone Remodelling Units (BRU) were found to be connected with BRUs inside callus around the defect; while in 10 mm model, such changes were not so obvious. Conclusion: In these tow different size model, we found that the cortical bone can directly involve in the bone healing process, in early stage by providing bone healing material, and in later stage by playing the role of remodeling the callus.
出处
《中国矫形外科杂志》
CAS
CSCD
2003年第7期460-462,共3页
Orthopedic Journal of China
基金
国家自然科学基金
香港大学郑裕彤奖学金资助(项目编号 :3980 0 1 65)
