摘要
目的 研究ICAM_1 LFA_1单抗阻断对口腔鳞癌肿瘤浸润淋巴细胞 (TIL)对自体肿瘤细胞杀伤活性及分泌TNF_α的影响。方法 从 15例口腔鳞癌组织中分离TIL并培养 ,用流式细胞术检测新鲜分离和激活的TIL细胞上ICAM_1和LFA_1的表达水平 ;比较用ICAM_1 LFA_1单抗阻断前后TIL对自体肿瘤细胞杀伤活性及产生TNF_α水平的影响。结果 激活的TIL较新鲜分离的TIL细胞ICAM_1和LAF_1的表达显著增加 (P <0 0 5 ) ;阻断ICAM_1,TIL对自体肿瘤细胞的杀伤活性无显著改变 ;阻断ICAM_1和LAF_1后 ,TIL分泌TNF_α无显著差异 (P >0 0 5 ) ,但TIL对自体肿瘤细胞的杀伤活性显著降低 (P <0 0 5 )。结论 anti_LFA_1单克隆抗体 (McAb)
Objective\ To investigate the effect of anti_ICAM_1/LAF_1 McAb on the cytotoxicity of tumor infiltrating lymphocytes from oral squamous cell carcinoma.Methods\ TIL were isolated from fresh tumor tissues in 15 patients with oral squamous cell carcinoma and cultured in vitro. The expression of ICAM_1/LAF_1 in fresh and activated TIL was examined by FACS. The cytotoxicity of TIL against auto tumor cells and the level of TNF_α produced by TIL were compared before and after interrupted with anti_ ICAM_1/LAF_1 McAb.Results\ The expression of ICAM_1and LAF_1 in the activated TIL significantly increased comparing with fresh TIL (P<0 05). Interrupted by anti_ICAM_1 McAb, the cytotoxicity of TIL against auto tumor cell was not significantly changed. Interrupted by anti_ICAM_1 and anti_LAF_1 McAb, the level of TNF_α produced by TIL showed no differences comparing with the control (P<0 05), but the cytotoxicity of TIL against auto tumor cells significantly decreased (P<0 05).Conclusion\ The effect of anti_LAF_1 McAb may result from steric interference of antigen_nonspecific adhesion process.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第2期107-108,111,共3页
West China Journal of Stomatology
