摘要
用^(125)Ⅰ固相放射免疫分析法测定了22头母鲁西黄牛的产后血浆孕酮(P_4)浓度。结果表明,在产后13.6±4.4天以前,母牛的血浆P_4浓度处于基础水平(0.34±0.04—0.55±0.06ng/ml,P<0.05),此后开始升高,母牛出现卵巢话动。代表卵巢活动的P_4曲线可分为4种类型:Ⅰ型,在产后10.6±3.9天以后,曲线呈现3个正常的黄体周期;Ⅱ型,在产后13.6±4.4天以后,首先出现一个8.5天左右的短黄体周期,然后出现两个正常的黄体周期;Ⅲ型,在产后12.5±3.9天以后出现一个正常的黄体周期,接着出现一个短黄体周期;Ⅳ型,在产后12.5±2.5天以后,出现不规则的黄体周期。在黄体周期中,最低P_4水平为0.45±0.15—0.60±0.38ng/ml。最高为2.65±1.95—4.17±2.35ng/ml。本试验表明,间隔4天测定1次血浆P_4浓度,可准确地判定母牛的发情周期。固相RIA灵敏度高,准确性和重复性好、操作简便。
The blood samples were collected from 22 Luxi cattle,aged 2.5-8,from calving day (day 0) to 80 days postpartum at 4-day interval.Progesterone(P4)le.vels in plasma were determined by solid phase RIA.The results are summarized as follows;P4 levels in plasma of the cows remained at 0.34±0.04-0.55±0.06ng/ml before:10.6±3.9-13.6±4.4 days postpartum,then they began to rise and the ovaria activity.appeared.The Plasma P4 profiles of the cow.s can be classified into 4 types,characterized by (Ⅰ) 3 normal cycles;(Ⅱ) a short cycle followed by 2 normal cycles;(Ⅲ) a normal cycle followed by a short.cycle and a normal cycle;.and (Ⅳ) some irregular cycles respectively.The lowest and the highest P4 levels were 0.45±0.15-0.60±0.38 and 2.65±1.95-4.1.7±2.35ng/ml respectively in luteal cycles.It is also concluded that determining plasma P4 concentrations at 4day interval can precisely identify the oestrus cycles of cows,and that the solid phase P4-RIA have practical value for determination of plasma P4 concentration.
出处
《核农学报》
CAS
CSCD
1992年第1期57-61,共5页
Journal of Nuclear Agricultural Sciences
基金
国际原子能机构的合作项目
关键词
固相RIA
血浆孕酮
牛
黄牛
卵巢
Solid phase P4-RIA,plasma progesterone,postpartum,fe-male yellow cattle,ovarian activity
