摘要
本室建立了雄激素受体(AR)测定的放射配基结合分析(RBA)法。测得人前列腺组织中胞浆、胞核的AR均可被约5nmol/l的双氢睾酮饱和,其最大结合容量分别为129.4fmol/mg胞浆蛋白和176.2fmol/mg胞核蛋白;平衡解离常数分别为0.88nmol/l和3.2nmol/l。人前列腺癌的胞浆胞核AR含量均高于正常前列腺及前列腺肥大的(P<0.05)。为前列腺癌病人选择治疗方案提供了理论依据。同时用融裱法放射自显影证实了AR主要分布在前列腺组织的腺区,尤其是腺上皮细胞核。
The result of radioligand binding assay study demonstrated that in the prostate both cytoplasmic and nuclear AR can be saturated by 5 nmol/1 dihydrotestosterone (DHT). The values of Bmax and KD are 129.4 fmol/mg protein and 0.88 nmol/1 in cytoplasm; 176.2 fmol/mg protein and 3.2 nmol/1 in nucleus. Both the cytoplasmic and nuclear AR content in prostatic cancer are higher than that in normal prostate and benign prostatic hypertrophy (P<0.05). It is useful in selecting suitable therapy for PC patients. Thaw-mount autoradiography demonstrated that AR are mainly located in the epithelial cells of the glandular tissue of prostate.
出处
《核技术》
CAS
CSCD
北大核心
1992年第5期257-261,共5页
Nuclear Techniques
关键词
雄激素受体
RBA法
前列腺癌
Androgen receptor(AR) Prostate Radioligand binding assayAutoradiography
