摘要
用免疫金电镜技术和电镜原位杂交技术观察了犬传染性肝炎病毒感染的犬肾传代细胞中的病毒包涵体,发现这些包涵体具有以下三种基本形态:1.松散均质包涵体;2.副结晶色包涵体;3.致密颗粒包涵体。其中前二种包涵体能被免疫金标记,它们是尚未成病毒粒子的病毒蛋白或是病毒装配后乘余的病毒蛋白,后一种包涵体能被病毒DNA探针标记,是病毒核酸合成过剩而堆积起来形成的产物。此外,本文还描述和讨论了包涵体与细胞及病毒发育成熟的关系。
Morphological characterization of Infectious canine hepatitis virus specific inclusion bodies was carried out by both immunogold electron microscopy and electron microscopy in situ hybridization. Three morphological types of inclusion bodies were observed: loose homogeneous, paracrystalline and dense granular inclusion body. The former two labelled with immunogold are viral antigenic protein of viral particles, the latter one labelled with viral DNA probe is composed of viral DNA. The possible relationship among the inclusion bodies and their bearing to virus morphogenesis were discussed.
出处
《中国病毒学》
CSCD
2003年第2期152-154,共3页
Virologica Sinica
关键词
犬传染性肝炎病毒
形态发生学
包涵体
免疫金电镜技术
电镜原位杂交技术
Infectious canine hepatitis virus
Inclusion body
Morphogenesis
Immunogold electron microscopy
Electron microscopy in situ hybridization
