摘要
目的 培养建立耐药肺癌细胞模型D6 /MVP并研究其生物学特性。方法 应用人肺癌细胞株D6 (简称D6细胞 ) ,采用MVP(MMC +VDS +DDP)大剂量间歇诱导法 ,建立耐药人肺癌细胞模型D6 /MVP(简称D6 /MVP细胞 ) ,观察该细胞的生长规律 ;用MTT法鉴定其对多种抗癌药物的多药耐药性 ;以流式细胞技术检测其细胞周期分布、细胞表面多药耐药基因 (MDR)的表达产物P 糖蛋白 (P gp)、多药耐药相关蛋白 (MRP)及谷胱甘肽硫转移系统 (GSH/GST)的表达等。结果 经MTT法鉴定 ,D6 /MVP细胞较D6细胞的MVP半数致死浓度 (IC50 )增大 13.3倍。经流式细胞仪检测 ,D6 /MVP细胞倍增时间明显延长 ,S期细胞减少 ,G2 期细胞增多 ;P gp和MRP的表达显著升高 (P <0 .0 1) ,而GSH/GST的表达无明显变化 (P <0 .0 5 )。结论 D6 /MVP细胞是一个明确的多药耐药细胞模型 ,具有耐药细胞的基本生物学特性。
Objective To establish human multidrug resistant lung carcinoma cell line (D6/MVP) with its characteristics studied. Methods Intermittent administration of high dose MMC, VDS and DDP (MVP) was used to induce human lung carcinoma cell line (D6) to a multidrug resistant variety (D6/MVP). MTT assay was used to study the multidrug resistance of D6/MVP to multianticarcinogen. Flow cytometry was used to study the cell cycle distribution and the expression of P gp, multidrug resistance associated protein (MRP) and GSH/GST. Results 1.D6/MVP was resistant to many anti tumor agents, with the IC 50 13.3 times higher and the drug resistance 2 6 times higher than D6, 2.The multiplication time of D6/MVP was prolonged and the cell number of S phase decreased while that of G1 and G 2 phase increased and 3.The expression of P gp and MRP was enhanced significantly (96.2% vs 51.7%), but the expression of GSH/GST kept stable. Conclusion D6/MVP is a multidrug resistant cell line possessing the basic characteristics of drug resistance.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2003年第2期134-136,共3页
Chinese Journal of Oncology
