摘要
目的 检测抑癌基因P16,P21和P53在骨巨细胞瘤(GCT)组织中的表达,探讨它们之间的相关性及与病理分级的关系,以进一步了解GCT的发生,细胞学性质及生物学行为,为临床治疗提供参考。方法 用免疫组织化学方法对49例存档GCT蜡块标本进行了检测。结果(1)P16、P21和P53 3种抑癌基因产物在GCT中均有表达,单核基质细胞和多核巨细胞均可着色,阳性率分别为71.4％、85.7％、38.8％,阳性细胞呈散在,片状或团块状分布。(2)P53在Ⅲ级与Ⅰ级和Ⅱ级之间的表达差异有显著性(P<0.01),P21在Ⅲ级与Ⅰ级之间的表达差异有显著性(P<0.01)。(3)P16和P21在GCT中的表达具有高度相关性(x^2=7.2,P<0.01),P16与P53,P21和P53之间的表达无相关性(x^2分别为2.728和3.593,P>0.05)。结论(1)GCT中存在两种单核基质细胞和多核巨细胞,部分单核基质细胞和少数多核巨细胞为肿瘤细胞,单核基质细胞为P53表达的主要肿瘤细胞成分。(2)病理分级能在一定程度上反映GCT的良恶性,结合P53与P21的检测可作为判断预后的指标。(3)P16蛋白与P21蛋白的表达呈高度相关性,P16蛋白与P53蛋白,P21蛋白与P53蛋白的表达没有相关性。
Objectives To investigate the expression of P16, P21 and P53 in GCT, to study the relationship among these three factors and to evaluate the features of cytology and oncogenesis and biological behavior of GCT. Method Forty - nine paraffin embedded specimens were examined by immunohistochemical method. Results CD The positive expression of all three genes including P16, P21 and P53 were observed in GCT and the positive rates were 71.4% , 85.7% ,38.8% respectively. The positive cells were scattering or massive in distribution (2) The positive expression of all three genes were seen in GCT with different histological grades. There was no significant difference among the three histological grades in P16, but remarkable significant difference between histological grade Ⅲ and Ⅱ or Ⅰ in P53 ( P < 0.01) and between Ⅲ and I in P21 (P<0.01) . (3) There was remarkable significant correlation between P16 and P21 (x2 =7.2, P < 0.01), and no significant correlation between P16 and P53(x2 =2.728 and 3.59, P > 0.05) . Conclusions (1) There are two kinds of stromal cells and multinucleated giant cells in GCT. Some stromal cells and a few multinucleated giant cells are tumor cells and positive stains of P53 are mainly in stromal cells. (2) The study suggested that Jaffe's classification can reflect malignancy of GCT, and with the detection of P53 and P21, the results can act as a index to help predict the prognosis of GCT(3) There was no significant correlation between P53 expression and P16 in GCT.
出处
《中国骨肿瘤骨病》
2003年第2期107-110,共4页
Chinse Journal Of Bone Tumor And Bone Disease
