摘要
应用毛细管气相色谱和气相色谱-质谱联用技术分析长江口海水悬浮物质中饱和及不饱和脂肪酸。结果表明,该技术具有高分辨率和灵敏度,测试的精密度和准确亦较好。该法一次测定可分别得到饱和、不饱和及支链脂肪酸在海水悬浮物中的含量,适用于海洋有机生物地球化学研究。
Seawater samples were immediately filtered on board ship with lμm glass fibres and obtained suspended matter samples from Changjiang Estuary seawate were kept frozen until analysis in laboratory. Before extraction, suspended matter were freeze-dried. Lipids were extracted in a soxhlet apparatus using 5:2 mixture of dichlormethane and methanol. Internal standard was added to each extract in order to evaluate the efficiency of the following analytical steps. The extract was saponified by 2 mol/L KOH-CH3OH and fatty acids mixtures were extracted. Fatty acids were methylated using 14% BF3 in methanol. Fatty acids esters mixtures were isolated with thin layer chromatography to get monofatty acids which were identified by GC-MS-DS. Determination of fatty acid methyl esters was performed by capillary gas chromatography. The method of internal standard was used in quantitative analysis. Data were corrected for losses during laboratory work-up, using recovered internal standard Analytical results was satisfactory.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1992年第6期669-676,共8页
Oceanologia Et Limnologia Sinica
关键词
悬浮物
脂肪酸
气相色谱
海水
Suspended matter, Fatty acids, Gas chromatography.
