摘要
应用常规显微和亚显微技术观察和分析患淋巴囊肿病养殖牙鲆(Paralichthysolivaceus)的病理学变化,利用差速离心和蔗糖密度梯度离心技术分离其病原———淋巴囊肿病毒,并利用牙鲆鳃组织细胞系FG-9307为感染基质,观察淋巴囊肿病毒引起的细胞病理变化。结果表明,患病牙鲆的囊肿组织是一些淋巴囊肿细胞的集合体,这些囊肿细胞排列紧密,直径为10～100μm,细胞近圆形,细胞质内散布有大量的嗜碱性包涵体,且多数集中在细胞的边缘部分;囊肿细胞内含有大量病毒粒子,其衣壳外形呈六角或五角形,直径为150～230nm,大多数病毒粒子中央有一致密的核,核外周包围着一双层核衣壳,核衣壳的表面可见一圈把手样亚单位。以患病牙鲆囊肿物制备的上清液接种细胞,7d内未见细胞异常,经盲传2～3代后,细胞出现较明显的细胞病变效应。
The diseased flounders Paralichthys olivaceus with typical lymphocystis symptom were collected from a flounder farm in Rongcheng, Shandong Province. The pathologic and histopathologic changes of the fish and the morphology of the virus particles were observed under optic and electronic microscope. The results show that the cyst tissues of the infected flounder are actually constituted by the aggregation of many lymphocystis cells in tight order and the size of the lymphocystis cells ranges from 10 to 100 μm in diameter, nearly roundshaped. The basophilic inclusion bodies exist in the cytoplasm of the lymphocystis cell and most of them are located on the edge of the cytoplasm. The shape of the viruses are hexagon or pentagon with knoblike subunits on its capsid. Most of the virus particles are 150-230 nm in diameter. The lymphocystis virus was isolated by inoculating the FG9307 with tissue homogeneous from infected fish, and after second and third blind passage, the virus became to adapt to growing on the cell and the plaque formed at day 7 after the infection. The virus was purified with differential centrifugation followed by sucrose density gradient centrifugation, and most of the viruses presented in a layer with 30% of sucrose concentration.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第2期117-120,共4页
Journal of Fishery Sciences of China
基金
国家高技术研究发展计划资助项目(2001AA622060)
关键词
牙鲆
淋巴囊肿病
病理学变化
淋巴囊肿病毒
病原
Paralichthys olivaceus
lymphocystis disease
pathology
pathogen isolation
