摘要
分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。
The photosynthetic pigments three marine red algae and one blue-green alga have been isolated and characterized. These algae are of Polysiphonia urceolata, Phycodrys sp. , Porphyra yezoensis (Rhodophyta) , and Spirulina platensis. By column chromatography of hydroxyapatite, C-phycocyanin , R-phycoerythin and allophycocyanin were purified. C-phycoerythin from P. yezoensis and Phycodrys sp. were similar each other, but different from that of the bule-green alga S. platensis in molecular weights, absorbance and fluorescence properties. Allophycocyanin from P. yezoensis and S. platensis were a little distinct from each other in absorbance and fluorscence spectra. Five R-phycoerythin with different molecular weights were compred in their absorbance and fluorscence characterization. Chlorophyll a in S. platensis is much more than that in P. yezoensis and Phycodrys sp.
出处
《海洋科学》
CAS
CSCD
北大核心
1992年第6期39-44,共6页
Marine Sciences
基金
国家自然科学基金课题
关键词
红藻门
蓝藻门
光合作用
色素
Blue-green algae
Red algae
Photosynthetic pigments
