摘要
从患"白内障"病的牛蛙肝脏、心脏、眼睛等器官中分离到A 1 L、B 4 H、B 2 E3株细菌.各以108CFU/mL的浓度进行肌肉注射健康蛙,剂量为0.2mL.发现B 2 E分离菌可使实验蛙100%产生与自然发病相似的"白内障"症状,死亡率达70%,可确认该菌为"白内障"病的致病菌.该分离菌大小为(0.3～0.5)μm×(0.6～0.8)μm,短杆状,极生单鞭毛,革兰氏阴性,无芽孢,无荚膜,主要生理生化特性是葡萄糖产酸、产气;氧化酶、赖氨酸脱羧酶、精氨酸脱羧酶阳性;鸟氨酸脱羧酶阴性;能在TCBS培养基上生长、培养基呈绿色,但不能在6%NaCl蛋白胨水上生长.根据其主要生理生化特性,初步鉴定为气单胞菌(Aeromonassp.).药敏试验结果表明,该菌对链霉素等敏感,对氨苄青霉素、青霉素G、先锋霉素V等不敏感.
Three bacterial strains were isolated from eyes,liver and heart of the diseased bullfrogs suffering from 'caratact'.After injecting 0.2mL 108CFU/mL bacteria liquid to each bullfrog,the isolated bacteria 'B2E' caused 100 % bullfrogs appeared the same symptoms as the spontaneous,and mortality was 70 %.It is considered that the isolated 'B2E' is the pathogen of 'caratact' disease.The physiology and biochemistry test results showed that the isolated organism was gramnegative,short rod,measured 0.3～0.5μm in width and 0.6～0.8μm in length,one polar flagella;produced acid and air from glucose;oxidase reaction,lysine,ornithine were positive;aesculin was negative;growth on TCBS,showed green but not on 6 % NaCl.The isolate is thought to be identified as Aerxomonas sp. strain.The drug sensitivity tests showed that the isolated pathogen was sensitive to Streptomycin etc,but resistive to Ampicillin,Penicillin,Cefazolin.
出处
《集美大学学报（自然科学版）》
CAS
北大核心
2003年第1期8-11,共4页
Journal of Jimei University：Natural Science
