摘要
目的 探讨基质金属蛋白酶 (MMPs)及其抑制物 (TIMPs)在大鼠肾脏衰老过程中的作用。 方法 选用 3、12和 2 4月龄大鼠 ,采用免疫组化技术分别检测基质金属蛋白酶 2、9(MMP 2、9)、组织金属蛋白酶抑制物 1、2 (TIMP 1、2 )、转化生长因子 β1(TGF β1)等在不同月龄大鼠肾组织中的表达。 结果 TIMP 1、TIMP 2及TGF β1主要表达在肾小球、肾小管、间质及血管 ,并随增龄表达增强 (P <0 0 1) ;MMP 9、MMP 2主要表达在肾小管上皮细胞 ,随增龄表达无变化 ;TIMP 1与TGF β1与肾小球硬化面积有相关性 (r分别为 0 75 1、0 771,P <0 0 5 ) ;TIMP 1与TIMP 2与小管间质纤维化面积有相关性 (r分别为 0 783、0 76 6 ,P <0 0 5 )。
Objective To investigate the expression and role of matrix metalloproteinases(MMPs) and tissue type inhibitors of metalloproteinases(TIMPs) during the aging in the kidney of rats. Methods Immunohistochemical assays were applied to detect the expression of TIMP 1, TIMP 2, MMP 9, MMP 2 and transforming growth factor β1(TGF β1) in renal tissue of rats which were divided into 3 groups (n=8): 3 month old group (3m), 12 month old group (12m) and 24 month old group (24 m). Results The expression of TIMP 1, TIMP 2 and TGF β1 was mainly in the glomeruli, tubulointerstitial and blood vessels, and the levels of their expressions were increased with aging( P <0 01). MMP 9 and MMP 2 was detected in tubular epithelial cells and no change of their expression was found during the aging. TIMP 1 and TGF β1 showed positive correlation with glomerulosclerosis( r =0 751, 0 771 respectively, P <0 05), and TIMP 1 and TIMP 2 showed positive correlation with area of the interstitial fibrosis ( r =0 783, 0 766 respectively, P <0 05) . Conclusions The imbalance between the expressions of matrix metalloproteinases and tissue type inhibitors of metalloproteinases may play an important role in the process of kidney aging.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2003年第4期228-232,共5页
Chinese Journal of Geriatrics
基金
"973"项目资助 (G2 0 0 0 0 5 70 0 3 )
国家自然科学基金"创新研究群体"项目资助 ( 3 0 12 10 0 5 )
