摘要
目的 建立转 bcl- x L 基因小鼠 ,继而传代建系 ,用于缺血性脑血管疾病的研究。方法 采用显微注射法 ,将人 bcl- x L 基因注入昆明小白鼠受精卵获得子代鼠 ,然后作 PCR、Southern- blot、m RNA、Western- blot检测以获得阳性鼠。结果 实验中注射受精卵 16 5 4枚 ,移植卵数 14 4 8枚 ,受体鼠 4 9只 ,怀孕鼠 7只 ,子代鼠 13只 ,整合 4只并均有表达。注射受精卵的存活率 87.5 4 % ,受精卵的总存活率 0 .78% ,受体鼠的怀孕率 14 .2 8% ,整合效率 30 .77% ,总有效率为 0 .2 4 %。结论 获得了转 bcl- x L 基因小鼠 ,但转基因的效率较低 。
Objective To develop bcl xL transgenic mouse and produce its lineages for studying ischemic cerebrovascular diseases. Methods Bcl xL gene was introduced into mice by microinjection. The presence of bcl xL and its expression were confirmed by PCR, Southern blot, mRNA inspection, Western blot in the founders. Results In our experiments, bcl xL gene was injected into pronucleus of 1654 zygotes and 1448 injected eggs were implanted into 49 foster mothers, 7 of which were pregnant and bore 13 offsprings. Among the babies, 4 offsprings were integrated with the bcl xL gene with the detectable expression. Totally, implanted zygotes' survival rate was 87 54 %, zygotes' total survival rate was 0 78 %, recipient mouse pregnance rate was 14 28 %, integration rate was 30 77 %, expression rate was 100 % and, total efficiency was 0 24 % respectively. Conclusion Bcl xL transgenic mice ( n =4) were obtained, but the transgenic efficiency was very low and the expression was unstable.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期180-184,共5页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金面上资助项目(No.39770 810 )
国家自科科学基金重点资助项目 (No. 39730 170 )
