摘要
目的 :建立检测可溶型TRAIL的ELISA ,并评价其临床应用价值。方法 :采用本室制备的鼠抗TRAIL的单抗 (mAb)FMU1.1作为包被抗体 ,兔的抗TRAIL高效价多抗为夹心抗体 ,建立检测sTRAIL的ELISA ,并测定了 9例肾综合征出血热 (HFRS)患者和 4 0例银屑病患者血清sTRAIL的水平。结果 :建立了由单抗和多抗组成的检测人sTRAIL的夹心ELISA ,灵敏度为 0 .0 8μg/L。 9例肾综合征出血热 (HFRS)患者中有 3例急性期患者血清sTRAIL水平升高 ,4 0例银屑病患者中 8例升高。结论 :成功地建立了一种可用于检测血清sTRAIL的双夹心ELISA ,为判定某些相关疾病患者的病情。
AIM: To establish quantitative ELISA for detecting souble TRAIL and to evaluate its clinical application. METHODS: The mAb FMU1.1 against TRAIL was used as coating antibody, and the rabbit polyclonal antibody to TRAIL as a sandwich antibody. The serum sTRAIL levels of nine patients with hemorrhagic fever with renal syndrome and forty psoriasis patients were measured by this method. RESUITS: Sandwich ELISA was established by combination of mAb and pAb. The sensitivity of the ELISA was 0.08 μg/L. The increased levels of serum sTRAIL were found in three cases out of nine HFRS patients and eight cases out of forty psoriasis patients. CONCLUSION: A sandwich ELISA is developed, which provide an useful tool judging the patients condition, curable effect and prognosis of some related diseases.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2003年第2期190-191,共2页
Chinese Journal of Cellular and Molecular Immunology
基金
国家重点基础研究发展规划(973)资助项目(No.2 0 0 1CB51 0 0 0 4 )
