摘要
以选出的A.niger2363—2~#为柠檬酸发酵用试验菌,研究了糖源、糖浓度、氮源、氮浓度、培养初始pH值、培养温度,培养基灭菌前与后调pH值等因素对其产酸的影响,并确定了其最佳培养条件。实验发现培养基灭菌后调pH值优于灭菌前调。
The production of cirtric acid by Aspergillus niger 2363-2~# was studied. Some important factors affecting the production of citric acid were investigated, involving carbon sources and concentration, nitrogen sources and concentration, pH, cultural temperature etc. The optimal conditions for citric acid production were determined as follows: sucrose concentration 8-14%, NH_4Cl 3.0-4.0 g/l, pH2.0, cultural temperature 30℃. It was also found that it is better to adjust the pH of the fermentation medium after autoclaving than before autoclaving.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
1992年第6期85-90,共6页
Journal of Harbin Institute of Technology
关键词
黑曲霉
发酵
柠檬酸
微生物培养
Aspergillus niger
fermentation
citric acid
citric acid fermentation microbial incubation
