摘要
将一１２ａａ的ＣＴＢ表位连接于丙型肝炎病毒（Ｈｅｐａｔｉｔｉｓ Ｃ ｖｉｒｕｓ，ＨＣＶ）复合多表位抗原基因ＰＣＸ的５’端，再克隆于真核表达载体ｐｃＤＮＡ３中，获得重组质粒ｐｃＤＮＡ３／ＣＴＢ－ＰＣＸ，利用表达ＩＬ－１２的ｐＷＲＧ／ｍＩＬ－１２作为佐剂，肌注免疫小鼠后６Ｗ，用重组蛋白ＣＺ－ＰＣＸ加强免疫１次，第６Ｗ时叶检测到较高水平的抗ＧＺ－ＰＣＸＩｇＧ，最高滴度达１：１０３，与对照ｐｃＤＮＡ３／ＰＣＸ相比无显著升高，提示ＣＴＢ表位并无明显的增强ＰＣＸ 基因特异性体液免疫应答的作用。重组蛋白ＧＺ－ＰＣＸ可有效提高抗体水平，促进ＣＤ８＋Ｔ细胞增殖。免疫小鼠叶诱发钉对ＧＺ－ＰＣＸ融合蛋白的迟发性超敏反应（ＤＴＨ），免疫后小鼠体重正常，肝脾未见明显肿大，具有良好安全性。
A l2aa epitope of CTB Was cloned at the 5' terminus of a multiple-epitope HCV gene PCX of hepatitis C virus (HCV), then cloned into a eukaryotic pcDNA3 to construct pcDNA3/CTB-PCX then immunned mice with a mIL-12 expression vector pWRG/mIL- 12 as art adjuvant. After six weeks of immunization. a HCV specific recombined protein GZ-PCX was used to boost the immunized mice. The results showed the specific antibodies were detected at week six (the titer 1: 10~3). which did not show significant difference compared with that of pcDNA3/PCX group, indicating this CTB epitope was effectiveless in enhancing the specific immune responses of PCX gene. After boosted with GZ - PCX antigen, the level of anti-GZ- PCX raised dramatically and the ratio of CD8+T cell increased. The immunized mice induced delayed- type ttypersensibility (DTH)by GZ - PCX antigen as well. The body weight and weight of livers and spleens of immunized mice did not show abnormality compared with those of blank and other immunized groups.
出处
《热带医学杂志》
CAS
2001年第1期13-16,共4页
Journal of Tropical Medicine
