摘要
目的 :了解同型半胱氨酸 (HCY)对血管内皮细胞 (VEC)凋亡的形响。方法 :用不同浓度的HCY处理离体培养的大鼠VEC24h ,用末端脱氧核苷酸转移酶 (TdT)介导的荧光素d -uTP缺口末端标记方法 (TUNEL)原位检测细胞凋亡 ,以TUNEL指数 (TUNELI)表示凋亡。结果 :正常对照培养条件下 (2 %FBS)VEC经历低频凋亡 ,其TUNELI为5.8±2.5。培基中加入浓度为0.1mmol/L的HCY ,凋亡细胞数开始增加 (TUNELI为12.6±4.4) ,但差异无显著性 (与对照比P>0.05) ;培基中HCY浓度为0.5mmol/L时 ,凋亡细胞明显增加 (TUNELI为22.4±6.4 ,与对照比 ,P<0.05) ,当培基中HCY浓度增加到1.0mmol/L时 ,将近一半的VEC经历凋亡性细胞死亡 (TUNELI为44.5±11.2 ,与0.1mmol/LHCY组比 ,P<0.05)。结论 :HCY浓度依赖性诱导VEC凋亡 ,VEC凋亡异常可能是动脉粥样硬化 (AS)发生的重要原因之一。
Objective:To evaluate the effect of homocysteine(HCY)on apoptosis of the vasˉcular endothelial cell(VEC).Methods:After the VEC exposed to the increasing concentrations of HCY(0.1-1.0mmol/L)for24hours,the apoptosis of VEC was determined with terminal deˉoxynucleotide transferase(TdT)mediated-flurosceine d-UTP nick-end labeling(TUNEL).Results:VEC experienced low frequency of apoptosis,and TUNELindex(TUNELI)was5.8±2.5in the control medium with2%fetal bovine serum(FBS).When0.1mmol/L concentration of HCY added to the basal medium(2%FBS),TUNELI increased by12.6±4.4(vs that in control,P>0.05).The number of apoptotic cell death increased markedly and TUNELI was22.4±6.4(vs that in control,P<0.05)in the medium with0.5mmol/L HCY.When the conˉcentration of HCY increased to1.0mmol/L,almost half of the cultured VEC experienced apoptosis identified with TUNEL method(TUNELI44.5±11.2,vs that in control,P<0.05).Conclusion:HCY induced the apoptosis of VEC in a dose-dependent manner,The apoptosis of VEC induced by HCY may contribute to the development and progression of atherosclerosis.
出处
《天津医科大学学报》
2003年第1期53-55,共3页
Journal of Tianjin Medical University
