摘要
目的 :探讨冠心病 (CHD)病变范围与载脂蛋白E(apoE)基因多态性及血脂分布的关系。 方法 :用酚氯仿抽提核酸法从凝血块中分离DNA ,用多聚酶链式反应 限制性片段长度多态性 (PCR RFLP)方法对新疆乌鲁木齐地区维、汉两民族人群中 10 2例CHD患者和 5 1例对照组人群进行apoE基因多态性 (由ε2、ε3和ε4决定的E2 / 2、E3/ 3、E4 / 4、E4 / 2、E4 / 3和E3/ 2 )HhaI酶切研究。结果 :①CHD组apoE之ε2 ,ε3和ε4等位基因频率分别为 0 .0 735± 0 .2 15 7,0 .774 5± 0 .3117和 0 .15 2 0± 0 .2 4 16 ,1支病变组apoE之ε2 ,ε3和ε4等位基因频率分别为 0 .10 6 1± 0 .2 4 2 3,0 .75 76± 0 .35 6 2和 0 .136 4± 0 .2 2 6 1,2支病变组apoE之ε2 ,ε3和ε4等位基因频率分别为 0 .0 5 5 6± 0 .1992 ,0 .80 5 6± 0 .2 995和 0 .1389± 0 .2 5 6 7,3支病变组apoE之ε2 ,ε3和ε4等位基因频率分别为 0 .0 6 0 6± 0 .2 0 76 ,0 .75 76± 0 .2 82 9和 0 .1818± 0 .2 4 4 3,与正常对照组 (0 .196 1± 0 .30 13,0 .6 6 6 7±0 .36 97和 0 .1373± 0 .2 2 5 4 )比较 ,ε2明显减低 (P <0 .0 5 ) ,病变范围越大 ,ε2越低 ,但病变范围大小之间统计学无差异 ,ε3和ε4随着病变范围增大逐渐升高但统计学无显著差别 (P >
Objective:To examine the relation between extent of coronary heart disease (CHD) and apolipoprotein E gene polymorphisms with blood lipid. Methods:DNA was obtained from the 102 patients and from 51 controls. In order to determine the apo E genotypes, DNA was PCR amplified and digested with HhaI. The genetic polymorphism of apo E is due to three common alleles, ε2, ε3, ε4, at a single autosomal gene locus. These alleles determine the six phenotypes E2/2, E3/3, E4/4, E4/2, E4/3, and E3/2. Results:In CHD patients group, the frequency of theε2, ε3 andε4 are 0.0735 ± 0.2157 , 0.7745 ± 0.3117 and 0.1520 ± 0.2416 , respectively. In one vessel CHD patients group, the frequency of theε2, ε3 andε4 are 0.1061 ± 0.2423 , 0.7576 ± 0.3562 and 0.1364 ± 0.2261 , respectively. In two vessels CHD patients group, the frequency of theε2, ε3 andε4 are 0.0556 ± 0.1992 , 0.8056 ± 0.2995 and 0.1389 ± 0.2567 , respectively. In three vessels CHD patients group, the frequency of theε2, ε3 andε4 are 0.0606 ± 0.2076 , 0.7576 ± 0.2829 and 0.1818 ± 0.2443 , respectively. In the control group, the frequency of theε2, ε3 andε4 are 0.1961 ± 0.3013 , 0.6667 ± 0.3697 and 0.1373 ± 0.2254 respectively. CHD patients have significantly lowerε2 allele and slightly higherε3 orε4 allele frequency than controls. Extent of CHD is bigger, ε2 allele is lower, among the one , two and three vessel CHD, there is no significant differences in statistics. ε3 andε4 allele frequency increases with extent of CHD but no significant differences in statistics. Age, sex, smoking, history of diabetes mellitus, serum TG and Lp(a) values significantly increased in CHD patients. triglyceride, total cholesterol, low density lipoprotein cholesterol, lipoprotein(a) tended to increased and high density lipoprotein cholesterol tended to decrease from the one vessel to three vessels. Conclusion:ε2 allele is significant lower in CHD patients, ε3 andε4 alleles is lightly higher. ε2 allele reduces andε3 andε4 alleles increases with extent of CHD. Deletion of ε2 might be one of the risk factors for CHD in Xinjiang Uygur and Han population.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2003年第3期134-137,共4页
Journal of Clinical Cardiology
