摘要
目的 探讨载脂蛋白H(apolipoproteinH ,apoH)基因第 8外显子G10 2 5C(Try3 16Ser)多态性与长沙地区汉族人脑卒中的关系及其对血脂的影响。方法 采用PCR 单链构象多态技术和DNA直接测序法检测长沙地区汉族 2 60例脑卒中患者、2 0个脑卒中家系成员和 10 0名健康对照者的apoH基因第 8外显子G10 2 5C(Try3 16Ser)多态性 ;酶联免疫吸附法检测抗磷脂抗体水平 ;酶法测定总胆固醇 (TC)、甘油三酯(TG)、高密度脂蛋白胆固醇 (HDL C)、低密度脂蛋白胆固醇 (LDL C) ,免疫比浊法测定apoA I及apoB10 0 ,酶联免疫吸附法测定脂蛋白a血清水平。结果 长沙地区汉族人apoH基因G10 2 5C(Try3 16Ser)多态存在GG、GC两种基因型 ,脑卒中组及其各亚组G10 2 5C(Try3 16Ser)多态基因频率分布与对照组比较差异无显著性 (P >0 .0 5 ) ;分析不同基因型对血脂、脂蛋白水平的影响 ,发现脑梗塞组和对照组GC基因型血清TG水平均明显高于GG基因型 (P <0 .0 5 )。结论 长沙地区汉族人apoH基因G10 2 5C(Try3 16Ser)多态性可能与脑卒中的易患性无关 ,但与血脂代谢有一定关联。
Objective: To investigate the relationship between G1025C(Try316Ser)polymorphism in exon 8 of apolipoprotein H (apoH) gene and stroke and to evaluate the effect of G1025C(Try316Ser) polymorphism on plasma lipid levels in Changsha Hans. Methods: G1025C(Try316Ser) polymorphism in apoH gene was determined by PCR-single strand conformation polymorphism analysis and DNA sequencing in 100 healthy controls, 260 patients with stroke, and 20 stroke pedigrees. Serum antiphospholipid antibody (APA) levels were tested by enzyme-linked immunosorbent assay(ELISA). Plasma lipid levels were measured by routine methods. Results: No statistically significant differences were found in frequencies of genotypes and alleles of G1025C(Try316Ser) polymorphism between the controls and stroke patients. The serum levels of TG in the GC genotype of cerebral infarction patients and controls were markedly higher than those in GG genotype. Conclusion: There was no association between G1025C (Try316Ser) polymorphism and stroke in Changsha Hans. G1025C (Try316Ser) polymorphism was associated with plasma lipid metabolism in Changsha Hans.
出处
《中华医学遗传学杂志》
EI
CAS
CSCD
2003年第2期114-118,共5页
Chinese Journal of Medical Genetics
关键词
脑卒中
载脂蛋白H
多态性
血脂
DNA sequences
Genes
Lipids
Patient treatment
Plasmas
Proteins
