摘要
探讨气滞血瘀型大鼠胸主动脉内膜剥脱后内膜增生中 ,舒心益脉煎对血管平滑肌细胞凋亡及相关基因Fas抗原和Fas配体的影响。将 5 8只大鼠随机分为假手术组 10只 ,舒心益脉煎组和对照组各 2 4只。后两组大鼠行胸主动脉内膜剥脱术 ,舒心益脉煎组动物于术前 7d至术后 14d每日接受舒心益脉煎治疗。术后 14d处死动物 ,用免疫组织化学法测定Fas抗原与Fas配体在血管中的表达 ,原位末端标记法测定凋亡细胞。术后 14d血管新生内膜中有平滑肌细胞凋亡 ;舒心益脉煎显著增加新生内膜中Fas抗原与Fas配体的表达 ,增加血管平滑肌细胞凋亡 ,减少新生内膜面积。说明舒心益脉煎可能通过Fas系统调节动脉血管内皮损伤修复过程中血管平滑肌细胞凋亡 。
Aim To investigate the effect of Shuxin Yimai Jian (SXYMJ) on the apoptosis and the related gene Fas antigen and Fas ligand (FasL) of vascular balloon injury in thoracic aorta in rats with stagnancy of qi and blood stasis. Methods Fifty-eight rats were randomly divided into the sham group (n=10), SXYMJ group (n=24) and the control group (n=24). The thoracic aorta endothelium was denudated in the SXYMJ and control groups. SXYMJ was given from 7 days before operation to 14 days after operation in the SXYMJ group. Fas and FasL were detected by immunohistochemistry and the apoptotic cells were stained in situ by terminal deoxynucleotidyl transferase mediated-deoxyuridine triphosphate nick end labeling. Results Apoptotic cells could be detected in the smooth muscle cell (SMC) at the 14th day after operation. The expression of Fas and FasL in the neointima was increased by SXYMJ. The apoptosis of SMC was enhanced by SXYMJ, while the neointima area was attenuated. Conclusion SXYMJ probably regulates the apoptosis of SMC through Fas system and then inhibits the neointima hyperplasia.
出处
《中国动脉硬化杂志》
CAS
CSCD
2003年第1期41-43,共3页
Chinese Journal of Arteriosclerosis
