摘要
目的 通过观察高压氧 (HBO)对血管内皮损伤大鼠纤溶系统以及血小板的数量 (PL T)及另 2项参数的影响 ,以证实 HBO对血栓的发生、发展过程有否抑制作用。方法 将 4 4只大鼠随机分成 6个组 ,正常组 (N组 ,n=6 )、模型对照组 (M组 ,n=10 )、模型制作后 HBO治疗 5天组 (T1 组 ,n=7)和 10天组 (T2 组 ,n=7)以及高压空气治疗 5天组 (T3组 ,n=7)和 10天组 (T4 组 ,n=7)。血管内皮细胞(VEC)损伤动物模型均以皮下注射大剂量肾上腺素制成。测定其血浆中纤溶酶原激活物抑制物 (PAI)和组织型纤溶酶原激活物 (t PA)的活性以及 PL T和另 2项参数。结果 T1 组和 T2 组比 M组 PAI活性明显较低 ,t PA活性和 PL T显著较高 (P<0 .0 5 ,P<0 .0 1) ;T3组和 T4 组较 M组 PAI、t PA活性及 PL T变化不明显 (P>0 .0 5 )。结论 HBO可以促进血管内皮损伤大鼠纤溶活性的恢复 ,减少血小板的聚集 ,促进内皮细胞损伤的修复 ,有效地抑制血栓前状态 ,从而预防血栓的形成。
Objective To investigate if hyperbaric oxygen could inhibit the thrombosis by observing fibrinolysis system,platelet count(PLT)and another two parameters in SD rats with injured vascular endothelial cells(VEC).Methods 44 rats were randomly divided into 6 groups:normal group(group N, n =6);model group(group M, n =10);group T\-1( n =7)were treated by hyperbaric oxygen for 5 days after the model was made;group T\-2( n =7) were treated by hyperbaric oxygen for 10 days after the model was made;group T\-3( n =7) were treated by hyperbaric air for 5 days after the model was made;group T\-4( n =7) were treated by hyperbaric air for 10 days after the model was made.The rat model with VEC was injury established by injecting high dose of adrenaline subcutaneously.The tissue type plasminogen activator(tPA) activity,plasminogen activator inhibitor(PAI) activity,PLT and another 2 parameters were measured.Results PAI activity both in group T\-1 and in group T\-2 was significantly lower than that in group M( P <0 05,0.01).tPA activity and PLT both in group T\-1 and in group T\-2 were significantly higher than those in group M( P <0 05,0.01).PAI activity,tPA activity and PLT did not show marked changes in group T\-3 and group T\-4,in comparison with group M( P >0 05).Conclusions HBO could significantly promote the recovery of fibrinolysis activity,decrease platelet aggregation,improve the recovery of injured VEC,inhibit the prethrombotic state effectively and prevent thrombosis.
出处
《中华航海医学与高气压医学杂志》
CAS
CSCD
2003年第1期42-45,共4页
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
关键词
高压氧
血管内皮细胞损伤
纤溶系统
血小板
Hyperbaric oxygen Vascular endothelial cell injury Fibrinolysis system Platelet
