摘要
[目的 ]检测肺癌组织中DNA损伤修复酶MGMT、DNA PKcs和Ku的表达变化 ,探讨其表达和肺癌发生发展及临床病理特征之间的关系。 [方法 ]采用免疫组织化学方法 (LSAB法 )检测 1 0例肺部良性病变和 44例肺癌标本中上述修复酶的表达情况 (每一标本同时检测正常及肿块两个部位 )。同时还检测了肺癌组织中突变型P53蛋白的表达。[结果 ]随着肿瘤的进展MGMT的表达可被诱导增强 ,DNA PKcs和Ku的表达则降低 ,三种修复酶在肺肿瘤组织的表达均较良性肺组织低 ,差异有显著性。DNA PKcs和Ku的表达具有明显相关性。突变型P53蛋白阳性标本中MGMT蛋白的平均表达得分较P53蛋白阴性标本显著降低。DNA损伤修复酶的表达和病人的年龄、性别、组织学分型、分级无关。 [结论 ]DNA损伤修复酶表达的改变在细胞的恶性转变过程中至少起到了一定的作用 。
To detect the expression of DNA repair enzymes:MGMT,DNA PKcs and Ku in lung cancer and normal tissues to evaluate their roles in lung cancer progression. Immunohistochemical method(LSAB) was used to detect the expression of DNA repair enzymes and P53 in 44 lung cancers. Their expression was also detected in histological normal lung tissue from the same patient. 10 patients of lung benign pathological changes are investigated at the same time for comparison. The expression of MGMT was induced to increase during cancer progression,but the expression of other enzymes decreased. There was a trend in cancer tissues to be significantly lower than benign pathological changes to the three enzymes. A significant correlation was found between the degree of expression of DNA PKcs and Ku . The average score of MGMT expression in P53+group is significantly lower than P53-groups. The statistical analysis revealed no correlation between repair enzymes expression and patients ages,sex, histological types and grades. [Conclusion] The changed expression of DNA repair enzymes is at least contributing malignant changes. DNA repair enzymes maybe become the biomarkers of lung cancer susceptibility.
出处
《环境与职业医学》
CAS
北大核心
2003年第2期75-77,共3页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金重大项目 (编号 :39990 570 )
