摘要
目的 探讨端粒酶逆转录酶 (humantelomerasereversetranscriptase ,hTERT)mRNA的表达在膀胱移行细胞癌诊断中的价值及临床意义。方法 采用实时荧光定量PCR检测 5 5例膀胱移行细胞癌组织、2 8例癌旁组织及 9例正常膀胱组织中hTERTmRNA的表达情况。结合临床资料分析评价其表达与膀胱癌临床生物学行为的关系。结果 5 5例膀胱移行细胞癌中hTERTmRNA的阳性率为 92 .7% (5 1 5 5 ) ,2 8例癌旁组织中hTERTmRNA的阳性率为 17.9% (5 2 8) ,9例正常膀胱组织中没有表达。hTERTmRNA的表达与患者的年龄、性别、肿瘤数目、大小及病理分级无关 (P >0 .0 5 ) ,不同的临床分期与肿瘤发病情况间的差异有显著意义 (P <0 .0 1)。结论 hTERT mRNA的表达与膀胱移行细胞癌组织的恶性行为密切相关 ,提示hTERT在膀胱移行细胞癌的发生及恶化过程中或许发挥重要作用。hTERTmRNA是一种有价值的肿瘤分子标记物 ,运用实时荧光定量PCR对其表达的检测有助于膀胱移行细胞癌的早期诊断。
Objective To study the clinical significance of hTERTmRNA expression in bladder transitional cell carcinoma(BTCC).Methods hTERTmRNA was examined with real time fluorescence quantitative PCR in 55 cases of BTCC and 28 cases of surrounding normal tissues as well as in 9 cases of normal bladder tissue.The correlation between the expression of hTERTmRNA and the biological behaviour of BTCC was evaluated .Results The positive rate of hTERTmRNA was 92.7%(51/55) in BTCC and 17.9%(5/28)in surrounding normal bladder tissue while there was no expression of hTERTmRNA in normal bladder tissue.The expression of hTERTmRNA was not related to patients' age and sex,the size ,number and pathological grade of BTCC(P>0.05),but related to the tumour status and clinical stage(P<0.01).Conclusion It is suggested that hTERTmRNA ,which perhaps plays an important role in the genesis and malignant progression of BTCC,could serve as a potential molecular tumour marker so much that it could be helpful in early diagnosis of BTCC.with real time fluorescence quantitative PCR.
出处
《潍坊医学院学报》
2003年第2期102-105,共4页
Acta Academiae Medicinae Weifang
