摘要
目的 建立一个简便易行的动物模型及细胞毒性T细胞 (CTL)检测体系 ,为HCV感染中CTL作用研究奠定理论基础。方法 用SP2 O作为靶细胞 ,LDH检测HCV核心区多肽免疫BALB c小鼠的CTL活性。结果 HCV核心区多肽免疫BALB c小鼠后 ,可用LDH检测其CTL活性 ,CTL活性可被CPA10 ( 5～ 2 3位氨基酸 )增强及被CPA9( 3 9～ 74位氨基酸 )抑制。结论 LDH可稳定检测BALB
Objective To establish a simple animal model and the cytotoxic T cell(CTL) detecting system for the studies of the effects of CTL on HCV infection. Methods The CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV was detected with lactic dehydrogenase (LDH) by using SP2/O cells as the target cells. Results CTL activity in BLAB/c mice immunized by polypeptides in the core region of HCV could be detected with LDH. The activity could be enhanced by CPA10(5～23 aa) but inhibited by CPA9(39～74 aa). Conclusion CTL activity in BLAB/c mice can be detected stably by LDH.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2003年第4期298-300,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 ( 39770 6 82 )
