摘要
目的 探讨应用自体骨髓基质细胞为种子细胞的组织工程骨修复骨缺损的效果。方法 将兔自体骨髓基质细胞经淋巴细胞分离液分离培养、传代扩增后 ,与胶原海绵混合培养后植于兔桡骨干 1.5cm缺损处。 12周后处死动物 ,通过大体形态及X线、组织学检查进行评价。比较细胞和胶原海绵复合物移植与单纯胶原移植治疗骨缺损的疗效。结果 以组织工程骨移植于骨缺损处 ,骨缺损完全愈合 ,骨髓腔通畅。而单纯胶原移植对照组骨缺损未愈合 ,髓腔不通 ,与实验组有明显差别。结论 骨髓基质细胞是一种良好的骨源细胞 ,能定向分化成骨细胞 ,其成骨机制为膜内化骨和软骨内化骨。
Objective To investigate the efficiency of treating bone defect by tissue engineering of bone based on bone marrow stromal cells.Methods Rabbit bone marrow stromal cell was separated for cell culture and subculture was done to increased number of bone marrow stromal cell.A combined grafting material was made by bone marrow stromal cell and collagen.The mixture or collagen was used respectively to repair 1.5 cm segmental defects created in the radius of rabbits.The repair capability for each of the treatment was assessed physically, radiographically and histologically at 12 weeks after operation.Results The bone defects had bony union with trabeculate and cortical bone on the specimen with bone marrow stromal cell and collagen sponge.The bony healing could not been found in the control group.There was significant difference between the two groups.Conclusion Bone marrow stromal cell is a kind of good bony stem cell,which can be subdivided into bone cell.The mechanism of osteogenesis are both intramembranous and endochondral ossification .The tissue engineering in which the bone marrow stromal cell is used as functional cell has a optimal perspective outlook.It is a satisfied method to repair bone defects.
出处
《哈尔滨医科大学学报》
CAS
2003年第1期47-50,共4页
Journal of Harbin Medical University
基金
黑龙江省科技厅资助项目 ( 2 0 0 0 )
