摘要
在pH =6 86磷酸盐缓冲溶液中 ,采用电化学 (循环伏安法、微分脉冲伏安法和交流阻抗及数据拟合技术 )、粘度测定、电子吸收光谱和溴化乙锭 (EB)荧光分析法研究了 [Cu(phen) (H2 O) (L Met) ] + (phen =1,10 邻菲咯啉 ,L Met =L 蛋氨酸 )与小牛胸腺DNA的相互作用 .结果发现中心铜离子在循环伏安图上呈现 1对明显的准可逆氧化还原波 .当加入一定量的DNA时 ,配合物的氧化还原峰电流明显降低 ,扩散系数减小 ,电化学反应电阻增大 ,电子光谱的最大吸收峰明显红移 ,产生明显的减色效应 ,同时 ,配合物也能较大程度地猝灭EB DNA体系的荧光 ,说明 [Cu(phen) (H2 O) (L Met) ] + 与DNA的作用较强 。
The interaction between [Cu(phen)(H 2O)(L-Met)] +(phen=1,10-phenanthroline and L-Met=L-methionine) and calf thymus DNA in phosphate buffer (pH=6.86) has been investigated by electrochemical techniques(cyclic voltammetry, differential pulse voltammetry, ac impedance and its data fitting), viscosity measurements, electronic absorption and ethidium bromide (EB) fluorescene spectroscopy. A quasi-reversible redox wave for the central copper ion from the cyclic voltammograms is shown at a plantium electrode. It has been found that the values of the reduction peak current and apparent diffusion coefficient decreased significantly in the presence of DNA compared with that in the absence of DNA, and the electrochemical reaction resistance values increased, the maximal absorption peaks from absorption spectra are red-shifted and the intensity is weakened. At the same time, [Cu(phen)(H 2O)(L-Met)] + can remarkably quench the emission intensity of EB-DNA system. All experimental results indicate that the interaction of [Cu(phen)(H 2O)(L-Met)] + with calf thymus DNA is strong and the part intercalation is proved to exist.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2003年第2期245-250,共6页
Acta Chimica Sinica
基金
国家自然科学基金 (No.30 0 70 1 88)
广东省自然科学基金 (Nos.0 1 1 2 1 7
990 4 52 )
英国皇家化学会研究基金资助项目
