摘要
目的 探讨CD34+ 富集细胞和单个核细胞 (MNC)的体外扩增特性。方法 利用Min iMACS系统富集CD34+ 细胞 ,在相同条件下与同批MNC进行对照培养 ;观察了再次富选和MNC培养上清 (MNC SN)对CD34+ 富集细胞扩增的影响 ;并尝试了MNCCD34- 细胞的培养。结果 虽然CD34+ 富集细胞具有很高的扩增潜力 ,但在培养过程中 ,其集落密度和CD34 + 细胞含量却始终呈下降趋势 ,而MNC在培养中却出现了一个上升的趋势 ,集落密度和CD34+ 细胞含量分别由第 0天的 (4 12± 16 7) 10 5细胞和 (1.12± 0 .4 2 ) %增至第 7天的 (116 2± 5 6 6 ) 10 5细胞和 (4 .17± 1.4 4 ) % ;再次富选可以使培养过的CD34+ 富集细胞的总细胞和CD34+ 细胞扩增能力大大提高 ;MNCCD34- 细胞具有集落形成和转化为CD34+ 细胞的能力 ;MNC SN对CD34+ 富集细胞的集落形成有促进作用 ,而同时又对CD34+ 细胞有促分化作用。结论 CD34+ 富集细胞在体外大量扩增的同时存在大量分化 ,其在培养过程中产生的CD34-细胞对CD34+ 细胞的扩增有抑制作用 ;脐血MNC中大量的CD34- 细胞含有造血干 祖细胞 ,其分泌的细胞因子有促进CD34+ 细胞向较为成熟的集落形成祖细胞分化的作用。
Objective To explore the ex vivo expansion characteristics of selected CD 34 + cells and mononuclear cells (MNC). Methods CD 34 + cells were isolated from umbilical cord blood MNC by MiniMACS system, expanded under the same conditions as that for MNC. The effects of re isolation and the MNC supernatant (MNC SN) on the selected CD 34 + cells were investigated. And the CD 34 - cells of MNC were cultured ex vivo. Results In the culture of selected CD 34 + cells,both the colony density and the proportion of the CD 34 + cells declined continuously with the culturing, although they presented a high proliferation potential. However, in the culture of the MNC,from day 0 to day 7,the colony density and the proportion of CD 34 + cells were increased from 412±167/10 5 cells and(1 12±0.42)% to 1 162±566/10 5 cells and (4.17±1.44)%, respectively. It was found that both the total cells and the CD 34 + cells restored expansion potential by re isolating. CD 34 - cells of MNC had the ability to form colony and could transform to CD 34 + cells. MNC SN can promote colony forming ability and lead to CD 34 + cells differentiation at the same time.Conclusions In ex vivo culture, selected CD 34 + cells presented a high proliferation and differentiation potentials, and the CD 34 - cells produced during the cultivation had inhibition effect on CD 34 + cells expansion. CD 34 - cell population from cord blood MNC contained hematopoietic stem/progenitor cells and the cytokines secreted by CD 34 - cells could induce CD 34 + cells to more mature colony forming cells.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2003年第2期74-77,共4页
Chinese Journal of Hematology
基金
国家"8 63"计划生物领域项目 ( 10 2 12 0 8 0 1)
上海市现代生物与医药项目 ( 0 0 43 190 0 3 )
关键词
脐血
CD34^细胞
单个核细胞
体外扩增
Umbilical cord blood
CD 34 + Cell
Mononuclear cell
Expansion,ex vivo
